摘要
目的研究在兔颏舌肌内注射肉毒杆菌毒素(botulinum toxin,BTX)能否建立舌根后气道狭窄的阻塞性睡眠呼吸暂停低通气综合征(OSAHS)动物模型。方法以24只新西兰大白兔为实验动物,建立实验组和两组对照组,实验组行颏舌肌内注射BTX,第一对照组行颏舌肌注射生理盐水,第二对照组行股二头肌注射BTX。用药后均行动脉血氧饱和度(SaO2)监测,人工计算氧减饱和度指数(ODI4),采用配对样本t检验,以P<0.05作为动物模型成功建立的标准。结果实验组颏舌肌内注射BTX后动物出现明显缺氧症状,SaO2监测显示睡眠状态下出现间歇性低氧,其ODI4与对照组进行统计学分析具有显著性差异。结论运用颏舌肌注射BTX能够成功的建立兔舌根后气道阻塞的OSAHS动物模型,这一模型更符合OSAHS患者发病原因和自然病程。
OBJECTIVE To set up the OSAHS model using botulinum toxin（BTX）injection into the genioglossus of rabbits. METHODS Twenty four New Zealand White rabbits were randomly divided into 3 groups—one experimental group and two control groups. The experiment was carried out by injection BTX into genioglossus of experimental group animals,injection equal volume normal sodium（NS）into one control group and injection equal volume BTX into biceps femoris to the other control group. The SaO2 of all animals was monitored continuously and the times of oxygen desaturation index（ODI4）were calculated. The animal model was considered as successfully built if the P0.05 according to the results of student test. RESULTS The experimental animals had the signs of hypoxia,cyanosis. The animals had intermittent SaO2 decreasing,and the significant differences existed between the experimental group and two control groups（P0.01）. CONCLUSION With the method of injection BTX into rabbits＇ genilglossus,OSAHS animal model can be set up successfully and this kind of model is similar with the signs and symptoms of mankind OSAHS.
出处
《中国耳鼻咽喉头颈外科》
北大核心
2010年第9期485-487,共3页
Chinese Archives of Otolaryngology-Head and Neck Surgery
关键词
睡眠呼吸暂停
阻塞性
疾病模型
动物
肉毒毒素类
颏舌肌
Sleep Apnea
Obstructive
Disease Models
Animal
Botulinum Toxins
palatopharyngeal tensor muscle
