摘要
用电喷雾电离质谱(ESI-MS)和串级质谱(MS/MS),并结合紫外光谱、荧光光谱等方法,研究一种芳烷醇哌嗪类抗抑郁化合物SIPI5358与α-、β-、γ-环糊精(CD)制备得到的非共价复合物.质谱分析结果显示,SIPI5358分子可以和α-CD生成配合比为1∶1的非共价复合物,而与β-、γ-CD生成不同配合比的非共价复合物.串级质谱的结果进一步验证β-CD与SIPI5358非共价复合物的组成.用紫外光谱和荧光光谱实验对液相中非共价复合物的形成进行了辅助研究,结果均再次验证了非共价复合物的生成.荧光光谱实验测得SIPI5358与β-CD反应的生成常数Kf=3.45×103 mol.L-1.
Non-covalent complexes of α-,β-,and γ-cyclodextrin（CD） with the antidepressant compound SIPI5358 were investigated by electrospray ionization mass spectrometry（ESI-MS）,tandem mass spectrometry（MS/MS）,UV spectroscopy,and fluorescence spectroscopy.The ESI-MS experimental results revealed that SIPI5358 reacted with the α-,β-,and γ-CD to form complexes with different coordination numbers.The tandem mass spectra further confirmed the formation of non-covalent complexes of SIPI5358 with β-CD.Binding of the complexes was also confirmed by UV as well as fluorescence spectra.The formation constant Kf was determined by fluorescence spectroscopy to be 3.45×103 mol.L-1 for the complex of SIPI5358 and β-CD.
出处
《物理化学学报》
SCIE
CAS
CSCD
北大核心
2010年第10期2604-2612,共9页
Acta Physico-Chimica Sinica
基金
国家自然科学基金(30772623)
国家十一五项目重大新药创制(2009ZX09103-047)资助项目~~
关键词
环糊精
抗抑郁
非共价复合物
紫外光谱
电喷雾电离质谱
荧光光谱
Cyclodextrin
Antidepressant
Non-covalent complex
UV spectroscopy
Electrospray ionization mass spectrometry
Fluorescence spectroscopy
