摘要
不同浓度的合欢叶提取物对油菜菌核病菌(Sclerotinia sclerotiorum)菌丝生长及菌核形成的抑制试验表明,该提取物具有抑制菌丝生长及菌核形成的作用,且随着浓度的提高活性增强,当浓度为20 mg/mL时,对菌丝生长的抑制率为73.1%,对菌核形成的抑制率为100%。通过对提取物处理后菌丝形态显微观察,发现病菌菌丝出现形态异常甚至断裂现象,且培养液电导率增加,推测为提取物处理后菌丝细胞壁破裂、细胞质外渗造成。苗期盆栽试验发现,该提取物对油菜菌核病菌有明显的防治效果,当浓度为20 mg/mL时,防治效果达72.1%。
The study showed that the crude extracts from leaves of Albizzia julibrissin Durazz had inhibition effect against the mycelium growth and sclerotial formation of Sclerotinia sclerotiorum in a dose-dependent manner,with the inhibition rate 73.1% for mycelia growth and 100% for sclerotial formation at concentration of 20 mg/mL.The microscope observation showed that the hyphae of S.sclerotiorum were deformed after being treated with the plant extracts,leading to a cytoplasm exudation and the increase of electrical conductivity of the mycelium suspension of the pathogen.The control effect（72.1%） of the extracts（20 mg/mL） against the disease was demonstrated at the seedling stage through pot culture experiment.
出处
《植物病理学报》
CAS
CSCD
北大核心
2010年第5期511-516,共6页
Acta Phytopathologica Sinica
基金
国家科技部农业科技成果转化基金(083919n8100)
上海市蔬菜学重点学科基金(B209)
关键词
合欢
植物提取物
油菜菌核病菌
抑制作用
防治效果
Albizzia julibrissin Durazz
extracts
Sclerotinia sclerotiorum
inhibition
control effect
