摘要
目的建立生发酊质量标准。方法用薄层色谱法对处方中的补骨脂、闹羊花定性鉴别;高效液相色谱法定量分析处方中补骨脂素、异补骨脂素的含量。结果薄层色谱法鉴别了补骨脂、闹羊花;色谱柱:CAPCELLPAKC18(250mm×4.6mm,5μm),流动相:甲醇-水(50:50);检测波长:246nm;流速:1.0ml/min。补骨脂素在6.00～60.00μg范围内呈良好的线性关系该,线性回归方程为:Y=73364.32X-3.59,r=0.9999;异补骨脂素在6.56～65.64μg范围内呈良好的线性关系,线性回归方程为:Y=73454.85X+4.01,r=0.9999,平均回收率补骨脂素为101.17%,RSD为0.96%;异补骨脂素为99.49%,RSD为1.75%。结论该方法准确、灵敏度高,重复性好。
Objective To set up a quality standard for Shengfa Tincture.Methods Fructus Psoraleae and Naoyanghua in the prescription were qualitatively identified by thin layer chromatography.The octadecylsilane bonded column was used as the stationary phase,and methanol-water （50：50） as the mobile phase.The column was CAPCELL PAK C18（250 mm×4.6 mm,5 μm）,with a detection wavelength of 246 nm and a flow rate of 1.0 ml/min.Results The calibration curve of psoralen was linear over the range of 6.56-65.64 μg,and the equation of linear regression was Y=73 364.32X-3.59（r=0.9999）.The calibration curve of isopsoralen was linear over the range of 6.56-65.64 μg,and the equation of linear regression was Y=73 454.85X＋4.01（r=0.9999）,The average recovery of psoralen and isopsoralen was 101.17%（RSD=0.96 %） and 99.49% （RSD=1.75%）,respectively.Conclusion The method is reliable and accurate.
出处
《解放军药学学报》
CAS
2010年第5期427-429,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
关键词
生发酊
补骨脂素
异补骨脂素
薄层色谱法
高效液相色谱法
Shengfa Tincture
psoralen and isopsoralen
thin layer chromatography
high performance liquid chromatography
