摘要
目的：观察颐正活脉丸对HRS诱导人脐血管内皮细胞损伤的影响。方法：本研究用高脂兔血清（HRS）培养人脐静脉内皮细胞（EC）造成细胞损伤模型;同时分别加入颐正活脉丸提取液或抗氧化剂维生素E,观察EC中脂质过氧化物（LPO）含量以及EC利用外源性花生四烯酸（AA）合成前列环素（PGI2）的能力。结果：HRS使培养的EC中LPO含量显著增加,AA孵育液中6-Keto-PGF1a含量显著下降,且随着HRS浓度的增加,作用逐渐增强。结论：颐正活脉丸提取液在浓度2.5-20μg/mL时,有与维生素E相似的阻止HRS引起的EC中LPO含量升高和PGI2合成减少的作用。
Objective：To study the yizheng huomaiwan,s effect on injury of human umbilical cord blood vessel endothelial cells.Methods： In this research,cell injury model was made by cultivating human umbilical cord vein endothelial cells with HRS;at the same time,extraction liquid of Yizheng Huomaiwan and antioxidant pills vitamin E were added respectively;the content of LPO in EC and EC＇s capacity of synthesis PGI2 with exogenous arachidonic acid were observed.Results： HRS has made the content of LPO in EC increase dramatically,and the content of 6-Keto-PGF1a decrease greatly;What＇s more,with the increase in the concentration of HRS,its function has gradually strenthened.Conclustion： When extraction liquid of Yizheng Huomaiwan has reached such a concentration 2.5~20μg/mL,it has the similar function with vitamin E which can prevent the increase of LPO in EC caused by HRS and the decrease of PGI2 synthesis.
出处
《中华中医药学刊》
CAS
2010年第10期2108-2109,共2页
Chinese Archives of Traditional Chinese Medicine
基金
河南省教育厅自然科学基金资助项目(2008A360029)
