摘要
背景:干细胞可在一定条件下体外培养扩增、定向分化为多种类型成熟细胞系。临床上运用干细胞治疗骨软骨缺损,股骨头坏死,脊髓神经损伤等骨科疾病方面已取得了很多成果。目的:简要综述近年来干细胞相关研究,总结其在骨科基础研究与临床应用中的进展。方法:以stem cells,differentiation,cytokine,bone fracture,bone formation,cartilage defect,tissue engineering为检索词,检索Pubmed数据库(2000-01/2010-01),以干细胞,细胞因子,骨折,骨软骨缺损,肌腱损伤,皮肤损伤,组织工程为检索词,检索中国期刊全文数据库(2000-01/2010-01)。纳入与干细胞以及干细胞在骨科应用方面的基础和临床研究,排除内容陈旧及重复研究。计算机初检得到190篇文献,根据纳入排除标准,最终纳入24篇。结果与结论:作为组织工程种子细胞,目前干细胞应用广泛,已涉及医学多个领域,临床上运用干细胞治疗骨科疾病大量的体外实验已获成功。干细胞具有多向分化潜能,在一定条件下可以分化为成骨细胞、成软骨细胞、成肌细胞和脂肪细胞。而与相应的生物材料相结合,能修复骨、软骨、肌腱、肌肉等多种组织,很多已不仅局限于实验研究,而且试用于临床患者的治疗,并取得了满意的疗效。
BACKGROUND：Stem cells are immature cells that can proliferate and differentiate into multi-lineage adult cells under certain conditions in vitro.Stem cells for treating osteochondral defect, femoral head necrosis and spinal nerve injury have obtained many achievements in clinic.OBJECTIVE：To summarize the new progress of stem cells and its applications in orthopedics in recent years.METHODS：A compute-based online search of Pubmed database from January 2000 to January 2010 and China National Knowledge Infrastructure from January 2000 to January 2010 was performed using the key wods of ＂stem cells, differentiation, bone formation, cytokine, bone fracture, cartilage defect, tissue engineering＂ in English and ＂stem cells, cytokine, bone fracture, bone defect, tendon injury, skin destruction, tissue engineering＂ in Chinese.The experimental studies about stem cells and clinical applications in orthopedics were included.Repeated or out-of-date studies were excluded.Totally, 190 articles were collected and finally 24 were included according to inclusion and exclusion criteria.RESULTS AND CONCLUSION：As seed cells for tissue engineering, stem cells have extensive usage in vitro, which involves many domains along medical science.Amounts of experiments of stem cells for orthopaedic diseases have succeeded in vitro.Stem cells with multiple-directional differentiation potential can differentiate into osteoblasts, chondroblasts, myoblasts and adipocytes.Combined with corresponding biomaterials, stem cells can repair bone, cartilage, tendon and muscle.Many not only limited in experimental study, but also fit for clinical treatment and obtained satisfactory outcomes.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第36期6817-6820,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
