摘要
目的探讨姜黄素(Curcumin)对人喉癌Hep-2细胞的生长抑制及诱导凋亡作用。方法将Hep-2细胞用含不同浓度姜黄素(3、6、12.5、25μmol/L)的培养基分别培养24和48h,采用MTT法检测其对细胞增殖的影响;台盼蓝染色法检测其对细胞活力的影响;流式细胞术检测其对细胞周期分布及细胞凋亡的影响;DNA琼脂糖凝胶电泳检测其对细胞DNA的影响。结果姜黄素可明显抑制Hep-2细胞增殖,且呈时间-剂量依赖性;可明显降低细胞活力,且呈剂量依赖性;可使细胞阻滞在G2/M期,并诱导细胞出现典型的凋亡峰,凋亡率呈时间-剂量依赖性;能使细胞DNA形成典型的DNA-Ladder。结论姜黄素对人喉癌Hep-2细胞增殖及细胞活力具有显著的抑制作用,并能诱导Hep-2细胞发生凋亡。
Objective To investigate the inhibition of growth and induction of apoptosis of human laryngeal cancer Hep-2 cells by curcumin. Methods Hep-2 cells were cultured in media containing curcumin at various concentrations （3, 6, 12. 5 and 25 μmol / L）for 24 and 48 h separately. The effects on cell proliferation was determined by MTT method, on cell viability by trypan blue staining, on distribution of cell cycle and cell apoptosis by flow cytometry, and on cell DNA by DNA agarose gel electrophoresis. Results Curcumin inhibited the proliferation of Hep-2 cells significantly in a time-and dose-dependent manner, decreased the cell viability in a dose-dependent manner, arrested the cells at G2 / M phase and induced obvious apoptosis peaks as well as apoptosis rate in a dose-and time-dependent manner. Typical DNA-Ladder was formed in the cell DNA treated with curcumin. Conclusion Curcumin significantly inhibited the proliferation and viability, and induced the apoptosis of Hep-2 cells.
出处
《中国生物制品学杂志》
CAS
CSCD
2010年第10期1105-1108,共4页
Chinese Journal of Biologicals
基金
吉林省教育厅"十一五"科学技术研究项目资助课题(2008-432)
关键词
姜黄素
喉癌细胞
细胞增殖
细胞凋亡
Curcumin
Laryngeal cancer cell
Cell proliferation
Cell apoptosis
