摘要
通过反转录ＰＣＲ，经克隆得到１３４７ｂｐ的ＴｕＭＶＨ１的ＨＣ片段，并在大肠杆菌表达载体ｐＧＥＭＥＸ１中得到表达，产物为可融性蛋白，分子量约为８０ｋＤ。
The turnip mosaic virus Hangzhou isolate 1(TuMV H1),which infected Brassica napus more seriously than other TuMV isolates,was isolated in Hangzhou.The HC gene of TuMV H1 was cloned by reverse transcription PCR and inserted into pGEMEX 1 fusion expression vector.A fusion protein containing the HC protein that is Mr≈80 kD was expressed in Escherichia coli.
出处
《浙江农业学报》
CSCD
1999年第2期59-62,共4页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金
浙江省自然科学基金
