摘要
水稻长芒是从野生稻保留下来的性状,通过人工选择培育的现代品种一般都是无芒类型。到目前为止,还没有关于控制芒的基因精细定位和克隆的报道。本实验利用国家种质资源库中有芒的亲本SLG与无芒亲本日本晴构建回交近等基因系(NILs)群体,从BC4F2回交分离群体中,选择出有芒和无芒呈3∶1分离的群体,从中选择杂合BC4F2单株构建BC4F3定位大群体。利用分离群体分组混合分析法(BSA法),筛选均匀分布水稻染色体组上1 512对SSR标记,将芒基因定位在第3染色体RM6283和RM5685之间,命名为AWN3-1。通过设计和筛选多态性标记,进一步将AWN3-1定位在Y5和Y9标记之间,遗传距离分别为0.5和0.4 cM。这些结果为克隆AWN3-1奠定了基础。
Long-awn trait was preserved from wild rice（Oryza rufipogon）,but in modern,the awnless varieties were developed by breeder.Up to now,there was no report about fine mapping or cloning awn gene.We developed a backcross between long-awn SLG（Japonica） as donor came from National Germplasm Library and awnless Nipponbare（Japonica）,and selected a small BC4F2 population which segregation ratio of awn accorded with 3∶1 to develop a large mapping population with heterozygous BC4F2 plants.Using bulked segregant analysis（BSA）,from 1 512 SSR makers evenly distributed on rice genome,we rough mapped the awn gene between RM6283 and RM5685 on chromosome 3,named AWN3-1.After developed and screened new markers,AWN3-1 was fine-mapped between marker Y5 and marker Y9,which genetic distance was 0.5 and 0.4 cM,respectively.This work provided a useful help for cloning AWN3-1.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2010年第5期1-5,共5页
Journal of China Agricultural University
基金
国家"973"计划项目(2004CB117201)
国家"863"计划项目(2006AA10Z158
2006AA100101)
国家科技支撑计划项目资助(2006BAD13B01)
