摘要
目的:克隆辽东楤木鲨烯合成酶基因并构建其植物表达载体。方法:以辽东楤木为材料,采用RT-PCR的方法,设计特异引物,克隆鲨烯合成酶基因,并将该基因连接到植物表达载体pBI121上。结果:获得辽东楤木鲨烯合成酶基因,该基因cDNA全长为1 261 bp,含有1 245 bp的开放阅读框(ORF),编码414个氨基酸,GeneBank登录号为GU354313。将AeSS基因定向克隆到植物表达载体pBI121的35 S启动子下游,构建了该基因的植物表达载体pAeSS。结论:首次获得了辽东楤木鲨烯合成酶基因并构建了该基因表达载体,为下一步的转基因研究奠定基础。
Objective： To clone Aralia elata squalene synthase gene（designated as AeSS）and construct plant expression vector for transgenic research.Methods： Isolated squalene synthase from Aralia elata with specific primers by RT-PCR and inserted AeSS gene into the plant expression vector pBI121.Results： The full-length cDNA of AeSS（Genebank accession Number： GU354313）was 1 261 bp and contained a 1 245 bp open reading frame（ORF）encoding a polypeptide of 414 amino acids.The plant expression vector pAeSS was constructed by inserted AeSS gene into the downstream of 35 S promoter of plant expression vector pBI121.Conclusion： AeSS gene was cloned and plant expression vector was constructed for future research.
出处
《中药材》
CAS
CSCD
北大核心
2010年第9期1363-1365,共3页
Journal of Chinese Medicinal Materials
基金
长春市科技计划项目(08YJ29)
关键词
辽东楤木
鲨烯合成酶基因
植物表达载体
Aralia elata（Miq.） Seem.
Squalene synthase
Plant expression vector
