摘要
目的:探讨腹膜高发性的人神经母细胞瘤细胞巨噬细胞移动抑制因子表达及对肿瘤发生发展的作用机理,为该病早期诊断及治疗寻找新的靶点。方法:RT-PCR分析人神经母细胞瘤细胞MIF mRNA表达,3H-TdR掺入法检测肿瘤细胞增殖,MTT实验分析NK细胞的对肿瘤细胞的杀伤活性,放射免疫分析法测定肿瘤细胞产生IL-10水平。结果:腹膜神经母细胞细胞表达MIF基因;基因重组MIF促进腹膜神经母细胞增殖,而抗MIF mAb抑制其增殖,二者均呈现剂量依赖性,与对照组相比,50ng/mL和100ng/mL组的差异最明显(P<0.05)。抗MIF单抗(50ng/mL)处理NK细胞12h和24h,可以明显抑制NK细胞对它的杀伤作用,尤其是效应细胞与靶细胞比例为50∶1和25∶1时最为明显;而用抗MIF单抗(50ng/mL)处理腹膜神经母细胞瘤细胞24h,可以明显提高NK细胞的杀伤率,可以明显抑制SK-N-SH细胞产生IL-10(P<0.05)。结论:MIF可以通过促进腹膜神经母细胞瘤细胞的增殖和增强其对免疫监视功能的抵抗促进肿瘤的发展。
Objective： To investigte the effects of macrophage migration inhibitory factor on neuroblastoma cell activity which was high occurred at peritoneum to explore the new target for diagnosis and treatment of neuroblastoma. Methods： RT-PCR was used for MIFmRNA analysis;3H-TdR incorporation assay was used for cell proliferation assay;NK cells kill activity was analyzed by MTT method;IL-10 level was analyzed by Radioimmunoassay. Results： MIF mRNA was expressed by neuroblastoma cell lines SH-N-SY. Recombinant MIF could enhance tumor cell prolifer-ation,while anti-MIF mAb could inhibit its proliferation,in a dose-dependent manner;Compared with control group,50 and 100 ng/mL group（both rMIF and anti-MIF mAb） had more obvious effects（P0.05） . NK cells killing activities was obviously inhibited by treatment with anti-MIF mAb（50ng/mL） for NK cells 12 h and 24 h（E： T 50：1,25：1） ,while treatment with anti-MIF mAb（50ng/mL） for neuroblastoma cells 24 h（E： T 50：1） ,NK cells killing activities was apparently increased. Compared with controls,treatment of neuroblastoma cells with anti-MIF mAb,24h cell,can obviously inhibit neuroblastoma cells to produce IL-10（P0.05） . Conclusion： MIF may play an important role in the development of neuroblastoma through promoting tumor proliferation and enhance its resistant to immune surveillance.
出处
《中国现代普通外科进展》
CAS
2010年第7期514-517,522,共5页
Chinese Journal of Current Advances in General Surgery
基金
山东大学2009大学生创新课题(2009282)
