摘要
目的:提取桃叶鸦葱根中多糖并测定多糖的含量。方法:采用煎煮法提取桃叶鸦葱根中多糖,采用苯酚-硫酸法测定多糖的含量。结果:煎煮法提取桃叶鸦葱根中多糖的提取率为13.9%。葡萄糖在所试浓度范围(0.03～0.08mg.mL-1)内线性关系良好,r=0.999 2。平均回收率为101.7%,RSD=0.61%。桃叶鸦葱根中多糖平均含量96.64 mg.g-1,RSD=0.10%。结论:苯酚-硫酸法测定桃叶鸦葱根中多糖含量方法稳定可行,简便。
Objective：To extract Polysaccharides from Scorzonera sinensis Lipsch,Et Krasch and determine the content of polysaccharides.Methods：To extract Polysaccharides with decoction method.The contents of the Polysaccharides in Scorzonera sinensis Lipsch,Et Krasch were determined by Phenol-sulfurie acid method.Results：The extraction yield of Polysaccharides was 13.9%,and the standard curve was excellent liner in the concentration range of 0.03～0.08 mg·mL-1 with correlation coefficient of r=0.999 2.The recovery rates was 101.7%,RSD=0.61%.The content of polysacharides from Scorzonera sinensis Lipsch,Et Krasch was 96.64mg·g-1,RSD=0.10%.Conclusion：The method is proved to be stable,feasible and simple.
出处
《河北北方学院学报（医学版）》
2010年第5期19-21,共3页
Journal of Hebei North University:Medical Edition
关键词
桃叶鸦葱
多糖类
分光光度法
Scorzonera sinensis
Polysaccharides
Spectrophotometry
