摘要
以‘冰糖橙’(Citrus sinensis‘Bingtang’)胚性愈伤组织为起始材料,进行了胚状体诱导和增殖培养。增殖过程中发现大部分胚状体出现畸形现象,具体表现为莲座型、玻璃化、白化或易愈伤化。为探究这些胚状体发生畸形的原因,比较研究了这4类胚状体部分可溶性物质含量和保护酶活性。结果表明,球形胚状体和易愈伤化胚状体的可溶性糖和蛋白质含量显著高于其他类型胚状体,白化胚状体的2种可溶性物质含量均最低;就SOD活性而言,球形胚状体>莲座型胚状体>易愈伤化胚状体>玻璃化胚状体>白化胚状体;就POD活性而言,玻璃化胚状体显著高于球形胚状体及其他畸形胚状体。研究结果为了解柑橘畸形胚状体发生的生理生化机制奠定了一定基础。
Embryogenic callus ofCitrus sinensis＇Bingtang＇was used as initial explants to explore embryoid induction and multiplication. A majority of embryoids were malformed during the course of the multiplication,such as rosette,vitrification,albino embryoids or embryoids tended to recallus readily. Contents of part of soluble matters and activity of protective enzymes were compared in this study to investigate the factors induced malformed embryoids. The results showed that the soluble sugar and protein in the globular embryoids and embryoids tended to recallus readily were significantly higher than those of others,respectively,and the least contents were in the albino embryoids. For the SOD activity,the globular embryoids〉the rosette embryoids〉embryoids tended to recallus readily〉the vitrified embryoids〉the albino embryoids. The POD activity of the vitrified embryoids was significantly higher than that of the globular embryoids and other types of malformed embryoids.
出处
《中国农学通报》
CSCD
北大核心
2010年第20期259-262,共4页
Chinese Agricultural Science Bulletin
基金
International Foundation for Science(IFS)项目(D/4756-1)
关键词
柑橘
愈伤组织
畸形胚状体
可溶性物质
保护酶
Citrus； callus； malformed embryoid； soluble matters； protective enzymes；
