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成年大鼠心肌缺血预处理的心肌保护作用及其对脂联素表达的影响 被引量：5

Ischemia preconditioning attenuated myocardial ischemia via upregulating the expression of adiponectin in rat
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摘要 目的 通过建立大鼠心肌缺血预处理（IPC）模型及心肌梗死模型,探讨IPC对心肌的保护作用及其对脂联素表达的影响.方法 成年大鼠（4月龄）建立IPC模型及IPC后心肌梗死模型,随机分为空白对照组（0、6、12和24 h亚组）、IPC组（0、6、12和24 h亚组）、单纯心肌梗死（MI）组及IPC＋MI组.采用Masson三色染色法测量心肌梗死面积（乳头肌平面梗死心肌与左心室心肌面积的比值,%）,免疫组织化学法和实时荧光定量PCR法检测脂联素在心肌组织中的蛋白和mRNA表达水平,利用大鼠血浆标本做酶联免疫吸附实验,观察循环中脂联素含量的变化.结果 IPC＋MI组大鼠心肌梗死面积明显小于MI组[（20±2）%比（31±3）%,P＜0.05＝.IPC组6和12 h亚组大鼠心肌组织中脂联素mRNA的表达分别是空白对照组相应时间点亚组的2.2倍和2.1倍（P均＜0.05＝.免疫组织化学结果 可见空白对照组及IPC组非缺血区心肌组织中脂联素未染色,而缺血的心肌组织局部脂联素染色加深,即脂联素在缺血区的表达明显高于非缺血区（P＜0.05＝.IPC组0、6和12 h亚组大鼠血浆中脂联素水平均显著高于空白对照组各相应时间点亚组（0 h：10.90±1.74比7.40±0.47 6 h：10.98±1.74比8.18±1.41 12 h：9.31±0.96比6.97±1.02,P均＜0.05＝.结论 IPC可减少心肌梗死的面积.IPC后心肌组织和血浆中脂联素表达水平均升高,提示脂联素参与了IPC的心肌保护作用. Objective To investigate whether adiponectin plays a role in the protection of myocardium in the rat myocardial ischemia preconditioning （IPC） model. Method Infarct size was measured by Masson＇s Trichrome staining, the expression of protein and mRNA of adiponectin at 0, 6, 12 and 24 h after IPC was examined by immunohistochemistry and quantitative real time RT-PCR, plasma levels of adiponectin at above mentioned four time points after IPC were detected by ELISA in IPC and MI rats. Result Infarct size was smaller in IPC than in MI rats （20% ±2% vs. 31% ±3%, P〈0. 05）. The expression of adiponectin mRNA at 6 h and 12 h after IPC was 2. 2 and 2. 1 times higher than in Sham rats at respective time points （P 〈 0. 05 ）. Immunohistochemistry staining evidenced increased adiponectin expression in the ischemic area and weak expression of adiponectin in non-ischemic area （ P 〈 0. 05 ）.Compared to the sham group, the plasma level of adiponectin increased significantly at 0, 6 and 12 h after IPC （0 h：7.40 ±0.47 vs. 10.90 ±1.74 6 h： 8. 18 ±1.41 vs. 10.98 ±1.74 12 h：6. 97±1.02 vs. 9. 31 ±0. 96, P 〈 0. 05 ）. Conclusion IPC reduced infarction size, upregulated the myocardial expression of adiponectin at mRNA and protein levels, and increased plasma adiponectin concentration, suggesting that the adiponecitn may play a critical role in the protective effect of IPC.
作者 王慧 程劲松 吴文静 温见燕 于长安 廖文强 孔炜 柯元南 郑金刚
机构地区 北京协和医学院研究生院 中日友好医院心血管病中心实验室 中日友好医院心内科 北京大学医学部生理与病理生理系
出处 《中华心血管病杂志》 CAS CSCD 北大核心 2010年第10期929-933,共5页 Chinese Journal of Cardiology
基金 基金项目：国家自然科学基金面上项目（30770865,30670849）
关键词 心肌梗死 缺血预处理 心肌 脂联素 Myocardial infarction Ischemic preconditioning,myocardial Adiponectin
分类号 R686 [医药卫生—骨科学]
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参考文献10

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同被引文献26

  • 1Hatano YM,Masatoshi I,Shizukiyo,et al.Plasma adiponectin level and myocardial infarction:the JMS Cohort Study[J].J Epidemiol,2009,19(2):49-55.
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  • 7Ohashi K,Ouchi N,Matsuzawa Y,et al.Adiponectin and hypertension[J].Am J Hypertens,2011,24(3):263-269.
  • 8Guo ZX,Zheng CH,Qin ZH,et al.Effect of telmisartan on the expression of cardiac adiponectin and its receptor 1 in type 2 diabetic rats[J].J Pharm Pharmacol,2011,63(1):87-94.
  • 9Eric E,Ouchi N,Wilson RM,et al.Adiponectin mediates cardioprotection in oxidative stress-induced cardiac myocyte remodeling[J].Am J Physiol Heart Circ Physiol,2011,301 (3):984-993.
  • 10Kazuhisa K,Rei S,Kazumasa U,et al.Impact of a single intracoronary administration of adiponectin on myocardial ischemia/reperfusion injury in a pig model[J].Circ Cardiovasc Interv,2010,3(2):166-173.

引证文献5

二级引证文献26

中华心血管病杂志
2010年 第10期

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