摘要
目的研究脂多糖(LPS)对大鼠星形胶质细胞Toll样受体表达的影响及其机制。方法在原代培养的第3代星形胶质细胞中加入不同浓度的LPS作用24 h,通过免疫荧光、Western blot观察星形胶质细胞Toll样受体的表达和NF-κB P65的表达,同时研究NF-κB通路抑制剂对其的影响。结果在正常状况下,星形胶质细胞在胞质和胞膜表达大量的TLR3受体及很少的TLR4受体。在LPS的刺激下,星形胶质细胞的TLR3表达保持不变,TLR4受体的表达随LPS量的增加而增高。LPS可刺激星形胶质细胞NF-κB的表达升高,抑制NF-κB通路活化可抑制TLR4受体的上调。结论星形胶质细胞Toll样受体的表达是不同源的,TLR4受体随环境的变化而改变,其分子机制可能与NF-κB信号途径有关。
Objective To investigate the expression of toll-like receptors in astrocytes administrated with lipopolysaccharide（LPS）.Methods LPS was administrated to rat astrocytes for 24 h,and the expression of toll-like receptors and NF-κB P65 in astrocytes was detected by immunofluorescence and Western blot.At the same time,the effect of inhibitor of NF-κB signal pathway was investigated.Results Astrocytes expressed both cell surface and intracellular TLR3,low-level TLR4 in normal circumstance.LPS up-regulated the expression of TLR4 and NF-κB P65 in astrocytes in a concentration-dependent manner,while the expression of TLR3 kept constant.Pretreatment with SN50（inhibitor of NF-κB） could block the increase of the expression of TLR4.Conclusion The expression of toll-like receptors in astrocytes was not homogeneous but rather tailored environmental signal.The molecular mechanism of the changes may be related to the expression of NF-κB P65.
出处
《基础医学与临床》
CSCD
北大核心
2010年第11期1206-1209,共4页
Basic and Clinical Medicine
