摘要
目的探讨红细胞生成素（ｒＨｕＥＰＯ）治疗慢性肾功能衰竭血液透析患者对血管的Ｎａ＋－Ｋ＋ＡＴＰａｓｅα１亚基表达的影响。方法选择２１例患者应用ｒＨｕＥＰＯ治疗，１７例应用输血治疗。采用ＲＴ－ＰＣＲ方法观察两组治疗后左桡动脉Ｎａ＋－Ｋ＋ＡＴＰａｓｅα１ｍＲＮＡ表达，同时测定治疗前、后红细胞膜Ｎａ＋－Ｋ＋ＡＴＰａｓｅ活性和颈动脉内膜一中层厚度。结果ｒＨｕＥＰＯ组Ｎａ＋－Ｋ＋ＡＴＰａｓｅα１ｍＲＮＡ表达上调，Ｎａ＋－Ｋ＋ＡＴＰａｓｅ活性升高，而输血组未见改变。结论ｒＨｕＥＰＯ能改善Ｎａ＋－Ｋ＋ＡＴＰａｓｅα１ｍＲＮＡ表达。
Objeetive:To
investigate the effects of recombinant human erythropoietin(rHuEPO)on the expression of
vascular Na + K +ATP ase α 1 subunit in hemodialysis patients. Methods:Anemia
was corrected by rHuEPO in 21 patients and by fresh blood transfusion in 17
patients.The targeted hematocrits(Hct)in both groups of patients were 30%.Relatively
quantitative RT PCR methods was used to detect Na + K +ATPase α 1 mRNA Levels in
left radial artery.Erythrocyte membrane Na + K +ATP ase activty and thickness of
intima middle of carotid were examined before and after the Hct correction.Result:The
expression Na + K +ATP ase α 1 mRNA was up regulated and activity of erythrocyte
membrane Na + K +ATP ase was markedly elevated after rHuEPO treatment.While in
transfused patients,no singnificant changs were detected in α 1 mRNA expression Na +
K +ATP ase activity. Conclusion:Treatment with rHuEPO improves expression of
vascular Na + K +ATP ase α 1 mRNA,whereas transfusion does not.
出处
《福建医药杂志》
CAS
1999年第1期12-14,共3页
Fujian Medical Journal
