摘要
目的:建立一种快速筛选和评价抗菌活性物质的方法,并将其应用于海洋抗菌活性物质的筛选。方法:在对生物样品的提取过程中引入加速溶剂萃取技术(ASE),同时采用刃天青作为指示剂间接判断不同提取物样品的抗菌特性。结果:建立了一种快速筛选抗菌药物的方法,并发现白棘三列海胆、梅氏长海胆、面包海星、秀丽节蛇尾的乙酸乙酯提取物对实验菌生长均具有较强抑制作用。结论:该方法克服了常规的纸片琼脂扩散法、试管肉汤稀释法等作为抗菌药物筛选方法的不足,可使数量庞大的生物活性物质中抗菌药物的高通量筛选与评价成为可能。
Objective： To establish a rapid screening and evaluating method for antibacterial active substance,and apply it to the screen of antibacterial marine active substance.Method： accelerated solvent extraction was applied during the active substance extraction procedure of biological samples,and then resazurin was used as a indicator to determine the antibacterial ability.Result： A rapid screening method for antibacterial medicine was worked out.What＇s more,we found that the ethyl acetate extract of Tripnetistes gratilla,Echinometra mathaei,Culcita novaeguineae and Ophiarthrum elegans have relatively strong inhibition to the experimental bacteria.Conclusion： It is an applicable method to realize high-throughput screen and evaluation of antibacterial biological-active substance,and overcome the shortage of conventional methods such as conventional methods.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第15期176-179,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家高技术研究发展计划项目(2007AA021500)
海南省自然科学基金项目(309001)
海南省重点科技计划项目(06202)
关键词
刃天青
抗菌
海洋药物
快速筛选
加速溶剂萃取
resazurin； antibacterial； marine drug； rapid screening； accelerated solvent extraction；
