摘要
目的研究Hedgehog信号通路中shh、ptch和smo蛋白过表达在乳腺浸润性导管癌及导管内原位癌中的临床病理学意义。方法采用免疫组化EnVision法检测111例浸润性导管癌、40例导管内原位癌和21例正常乳腺导管上皮组织芯片中shh、ptch和smo蛋白的表达情况,并分析其相关性。结果 shh、ptch和smo蛋白在正常导管上皮中表达呈阴性或弱阳性,但在浸润性导管癌(shh74.8%,ptch68.5%,smo65.8%)和导管内原位癌(shh100%,ptch100%,smo90%)中阳性表达率明显增高,差异有统计学意义(P均<0.001)。乳腺导管癌中shh蛋白的表达率在Ki-67和c-erbB-2阳性组明显高于阴性组(P均<0.05);而且与淋巴结转移相关(P<0.05)。此外,shh、ptch和smo蛋白表达与乳腺癌患者的年龄、ER、PR、p53蛋白的表达及组织学分级和临床分期等特征均无显著性。Spearman相关分析显示,在乳腺癌组织中shh的表达分别与ptch(r=0.55,P<0.01)、smo(r=0.58,P<0.01)的表达呈正相关;而且ptch的表达又与smo的表达呈正相关(r=0.495,P<0.01)。结论 shh、ptch和smo蛋白表达检测可以作为乳腺癌的辅助诊断指标,而且shh对判定乳腺癌的预后有一定作用。
Purpose To investigate the clinicopathological significance of shh,ptch and smo protein overexpression in breast invasive ductal carcinoma and ductal carcinoma in situ （DCIS）.Methods The expression of shh,ptch and smo proteins was detected by EnVision immunohistochemical method in tissue-microarray including 111 cases of breast invasive ductal carcinoma,40 cases of DCIS,and 21 cases of normal breast ductal epithelial tissues,and their correlations were also analyzed.Results The immunohistochemical staining showed that the expression of shh,ptch and smo in normal ductal epithelium were completely absent or rare,but significantly higher in invasive ductal carcinoma （shh,74.8%;ptch,68.5%;smo,65.8%）and DCIS（shh,100%;ptch,100%; smo,90%）（all P0.001）.In invasive ductal carcinoma,shh protein expression rate was significantly higher in both of Ki-67 and c-erbB-2 positive groups than negative groups（all P0.05）,and was correlated to lymph node metastasis（P0.05）.Whereas the expression of shh,ptch and smo in breast cancer showed no correlation to patients＇age,ER,PR,p53 protein expression,pathologic grade,and clinical stage.Spearman correlation analysis showed that shh expression was positively correlated to ptch（r=0.55,P0.01） and smo（r=0.58,P0.01）expression,respectively;and ptch expression was positively correlated to smo expression（r=0.495,P0.01）.Conclusions shh,ptch and smo protein overexpression might be a helpful marker for the diagnosis of breast cancer,and the detection of shh might be helpful in the evaluation of prognosis in patients with breast cancer.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2010年第5期518-522,共5页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金(30960403)
国家教育部留学回国人员科研启动基金(教外司留20071108)
关键词
乳腺肿瘤
Hh信号通路
免疫组化
breast cancer
hedgehog signaling
immunohistochemistry
