摘要
采用半定量RT-PCR分析重金属Cu、Cd在不同剂量以及不同处理时间下对鲫(Carassiusauratus)肝脏组织基因表达的影响。共检测了过氧化氢酶、热激蛋白、金属硫蛋白等17个逆境胁迫相关基因。9个Cu处理中,GSTα、GSTπ、CAT在鲫肝脏中诱导表达,其余基因的表达特性属于中间型,即在一些处理中表达上调,另一些处理中表达下调。Cd处理中,只有GSTα属于完全的诱导表达型基因。HSP30、GR以及GPx4a在Cd胁迫的鲫肝脏中表达受抑制,其余基因的表达属于中间型。虽然Cu、Cd胁迫对各基因转录水平的的影响不尽相同,多数处理中Cu诱导基因表达的程度略高于Cd。实验结果还显示,本研究设置的Cu、Cd处理剂量以及处理时间与鲫肝脏基因的表达变化之间没有明显的相关性。逆境胁迫相关基因在Cu、Cd处理鲫的肝脏中表达水平变化多样。本研究旨为深入研究鱼类基因表达与重金属解毒分子机制间的关系以及重金属毒理奠定了基础。
In this study the semi-quantitative RT-PCR was applied to detect gene expression in liver tissue of Carassius auratus after exposure to Cu2＋ or Cd2＋ with different dose for 12 h,24 h,and 36 h,respectively.Totally 17 stress-related genes were used to analyze the gene expression during stress of the two heavy metals including the catalase,metallothionein,heat shock protein genes,and so on.GST α,GST π and CAT were upregulated in liver of C.auratus throughout the experiments of Cu2＋ stress,and the expression of the other genes belonged to the middle type which meant that those genes were induced in some assays of heavy-metal stress but depressed in the other assays.GST α was the only one induced by exposure to Cd2＋.In contrast,mRNA abundance of HSP 30,GR,and GPx 4a were decreased evidently in liver tissue of C.auratus exposed to Cd2＋,and the expression of the other 13 genes fell into the middle type.Although exposure to Cu2＋ and Cd2＋ had diverse effects on the transcription level of the 17 genes,Cu2＋ induced higher gene expression than Cd2＋ did.There was no evident dose-dependent or time-dependent responses of these stress-related genes in liver of C.auratus following exposure to Cu2＋ and Cd2＋.The expression levels of stress-related genes greatly varied in liver of C.auratus treatment with Cu2＋ or Cd2＋,and the results of present study is helpful for further understanding the relationship between gene expression of fish and molecular mechanism of heavy metal detoxification.
出处
《中国水产科学》
CAS
CSCD
北大核心
2010年第6期1243-1249,共7页
Journal of Fishery Sciences of China
基金
国家"十一五"科技支撑计划项目(2006BAD01A1205)
现代农业产业技术体系建设专项资金资助(NYCYTX-49)
关键词
鲫
肝脏
基因表达
RT-PCR
铜
镉
Carassius auratus
liver
gene expression
RT-PCR
Cu
Cd
