摘要
背景:影响神经干细胞增殖分化的外在因素包括细胞因子和微环境,嗅鞘细胞能分泌多种细胞因子,与神经干细胞共培养时改变其微环境。目的:观察不同浓度嗅鞘细胞对神经干细胞增殖及分化的影响。方法:体外分别培养Wistar大鼠神经干细胞和嗅鞘细胞,5×107L-1神经干细胞分别和1×107L-1,1×109L-1,1×1011L-1嗅鞘细胞共培养,同时设立正常对照组,不加嗅鞘细胞。倒置荧光显微镜下观察神经干细胞增殖情况,诱导7d后行NSE免疫细胞化学染色,计算阳性细胞/细胞总数得出阳性细胞百分比。结果与结论:①3种浓度嗅鞘细胞与神经干细胞共培养3d后,均促进了神经干细胞增殖,以1×109L-1嗅鞘细胞共培养组作用最显著,明显优于1×107L-1嗅鞘细胞组、1×1011L-1嗅鞘细胞组。②神经干细胞与1×109L-1嗅鞘细胞共培养7d后,神经干细胞分化为神经元样细胞的百分比最高,与正常对照组相比差异有显著性意义(P<0.01)。
BACKGROUND：External factors that effect proliferation and differentiation of neural stem cells（NSCs） contain cytokines and microenviroment.Olfactory ensheathing cells（OECs） secrete a variety of cytokines and change the microenvironment of NSCs.OBJECTIVE：To investigate effects of different concentrations of OECs on the proliferation and differentiation of NSCs.METHODS：NSCs and OECs isolated from Wistar rats were cultured in vitro.5×107/L NSCs were separately cocultured with 1×107/L,1×109/L,1×1011/L OECs.Normal control group was set up,without OECs.The proliferation of NSCs was observed under an inverted fluorescent microscope.At 7 days after co-culture,the expression of neuron specific enolase was detected by immunocytochemistry.Ratio of positive cells to total cells was calculated.RESULTS AND CONCLUSION：At 3 days after co-culture,three different concentrations of OECs promoted the proliferation of NSCs.The effects were significant in the NSCs ＋ 1×109/L OECs group,compared with NSCs ＋ 1×107/L OECs group and NSCs ＋ 1×1011/L OECs group.At 7 days after co-culture,the percentage of neurons immunostaining cells was significantly greater in the NSCs ＋ 1×109/L OECs group,which showed significant differences compared with normal control group（P 0.01）.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第40期7501-7504,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
黑龙江省自然科学基金项目(D2008-64)~~
