摘要
背景:骨折不愈合严重影响患者生活质量,神经生长因子用于骨不连的防治是重建外科领域研究的重点和方向。目的:观察神经生长因子降钙素基因相关肽对失神经支配兔骨折愈合的影响。方法:构建大白兔失神经支配腓骨骨折模型后,实验组骨折端局部注射降钙素基因相关肽10μg,每2d一次;对照组骨折端局部注射等量生理盐水。术后2,4,6周分别测定静脉血中肾上腺素和去甲肾上腺素的水平,并进行生物力学和组织学分析。结果与结论:术后两组血清肾上腺素和去甲肾上腺素水平均有显著增高,在术后第4周达到最高峰,比术前高出1倍以上,第6周时出现下降,但仍然显著高于术前;术后2,4,6周,实验组肾上腺素和去甲肾上腺素血清水平较对照组显著增高,差异具有显著性意义(P<0.05～0.01)。在三点弯曲实验中,实验组骨痂的抗弯强度明显高于对照组(P<0.05～0.001);术后第6周时苏木精-伊红染色可见实验组骨痂中的透明软骨细胞已被成骨细胞替代,对照组骨痂中的软骨细胞仍没有完全成骨细胞化。提示神经生长因子降钙素基因相关肽对失神经支配兔骨折愈合具有明显的促进作用。
BACKGROUND： Bone nonunion affects life quality of patients severely, how to prevent and cure bone nonunion using nerve growth factor is the key point and direction in the reconstructive surgery field. OBJECTIVE： To study the effect of nerve growth factor （calcitonin gene-related peptide） on denervated fracture healing. METHODS： The rabbit models of denervation of fracture healing were established and divided into 2 groups. Totally 10 μg calcitonin gene-related peptide were injected into fracture end of rabbits in the experimental group, once two days. The same volume of physiological saline was injected into the control group. The serum concentrations of norepinephrine and epinephrine were mensurated at 2, 4, and 6 weeks after operation. Specimens of right fibula were sectioned to be studied by biomechanical test and histomorphometry study. RESULTS AND CONCLUSION： The serum concentrations of norepinephrine and epinephrine were dramatically increased in two groups after operation, reached a peak at 4 weeks, which was 1 fold of before operation, and then decreased at 6 weeks, but still higher than that of before operation. Compared with the control group, the serum concentrations of norepinephrine and epinephrine were higher （P0.05-0.01）. The three-point bending test showed that the bending strength of the experimental group was obviously greater than that of the control group （P0.05-0.001）. Hematoxylin-eosin staining showed that hyaline cartilage cells had been replaced by osteoblasts in the experimental group at 6 weeks after operation. No complete osteoblasts could be found in the control group. The results revealed that, calcitonin gene-related peptide can remarkably promote the fracture healing in denervated fracture healing rabbits.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第41期7675-7677,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
