摘要
目的:建立反相高效液相色谱法同时测定多种维生素注射液(13)中维生素A、维生素K1和维生素E含量的方法。方法:色谱柱:DiamonsilC18,流动相:甲醇,流速:2.0mL·min-1,检测波长:265nm。结果:维生素A、维生素K1和维生素E的检测浓度线性范围分别为60～240μg·mL-1(r=0.9997)、74～296μg·mL-1(r=0.9999)、4～16mg·mL-1(r=0.9998),平均回收率分别为99.9%、99.9%、100.1%(RSD=0.35%、0.32%、0.20%)。结论:该方法简单、快速、灵敏度高、重复性好,可同时测定多种维生素注射液(13)中维生素A、维生素K1和维生素E的含量。
OBJECTIVE： To establish RP-HPLC method for the content determination of vitamin A, vitamin K1, vitamin E in Multivitamin injection （13）. METHODS： The determination was performed on Diamonsil C18 column, with mobile phase consisted of methanol at a flow rate of 2.0 mL·min^-1 and the detection wavelength was set at 265 nm. RESULTS： The linear ranges were 60-240 μg·mL^-1 for vitamin A （r=0.999 7）, 74-296 μg·mL^-1 for vitamin K1 （r=0.999 9） and 4416 mg·mL^-1 for vitamin E （r=0.999 8）. The average recoveries were 99.9% （RSD=0.35%）, 99.9% （RSD=0.32%）, 100.1% （RSD=0.20%）. CONCLUSION： The method is simple, rapid, accurate and reproducible for simultaneous determination of vitamin A, vitamin K1 and vitamin E in Multivitamin injection （13）.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第45期4298-4299,共2页
China Pharmacy
