摘要
通过单因素试验,对红松SRAP-PCR反应体系的主要影响因子(Taq DNA聚合酶、Mg2+、模板DNA、dNTPs、引物浓度)进行了优化筛选,得出红松SRAP-PCR反应的最佳体系:20μL反应体系中,模板90 ng,Mg2+1.0 mmol/L,dNTPs 0.15 mmol/L,Taq DNA聚合酶1.5 U,引物0.3～0.4μmol/L。
Optimum selection were conducted on the major influenfial factors （Taq DNA polymerase, Mg^2＋, template DNA, dNTPs and concentration of primer） for the SRAP-PCR reaction systems of Pinus koraiensis by means of one-factor experiments, and the best SRAP-PCR reaction systems of Pinus koraiensis were worked out： in the 201aL reaction systems, there were 90 ng of template, 1.0 mmol·L^-1 of Mg^2＋, 0.15 mmol·L^-1 of dNTPs, 1.5 U ofTaq DNA polymerase and 0.3 - 0.4 μmol · L^-1 of primer.
出处
《辽宁林业科技》
2010年第6期6-8,共3页
Liaoning Forestry Science and Technology
基金
辽宁省科技厅攻关项目(2008207001)
辽宁省博士启动基金项目(20091037)
