摘要
目的:观察慢性浅表性胃炎、N-Ⅱb型肠化、小凹上皮增生、Ⅱb型肠化及异型增生胃黏膜细胞核内DNA含量以及细胞动力学的变化.方法:用FCM研究25例浅表性胃炎DNA含量及细胞动力学变化,并与萎缩性胃炎及癌旁组织中出现的N-Ⅱb型肠化,小凹上皮增生、Ⅱb型肠化及异型增生相比较.结果:有关细胞动力学参数提示由浅表性胃炎-萎缩性胃炎-癌旁组织,SPF,PI,G2M期分数逐渐增高(P<0.05).由浅表性胃炎-N-Ⅱb型肠化-小凹上皮增生-Ⅱb型肠化-异型增生,PI,SPF,G2M期分数逐渐增高(P<0.05).结论:潜在的癌变危险性可以用量化的细胞动力学分析,从观察细胞增殖运动状态可以推断其病变危险性.
AIM：To investigate the changes in DNA content and cell kinetics in different gastric mucosal lesions.METHODS：The DNA content and cell kinetics were investigated by flow cytometry in 25 superficial gastritis specimens and equal number of specimens of type N-IIb intestinal metaplasia,foveolar hyperplasia,type IIb intestinal metaplasia,and dysplasia taken from patients with chronic atrophic gastritis or gastric cancer.RESULTS：DNA histogram analysis revealed that proliferative index（PI）,S phase fraction（SPF） and G 2 M fraction were highest in dysplasia,followed by type IIb intestinal metaplasia,foveolar hyperplasia,type N-IIb intestinal metaplasia and superficial gastritis.CONCLUSION：Quantitative cytokinetic analysis can be used to evaluate the risk of malignant transformation of gastric lesions.
出处
《世界华人消化杂志》
CAS
北大核心
2010年第29期3147-3150,共4页
World Chinese Journal of Digestology
