摘要
目的观察丰富环境(EE)对快速老化(SAMP8)小鼠黑质脑源性神经营养因子(BDNF)的影响,探讨EE保护黑质多巴胺神经元降低1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)损伤的机制。方法 80只3个月龄雌性SAMP8小鼠随机分为EE组和标准环境组(SE组)。饲养3个月后,EE组和SE组小鼠分别随机分为MPTP组和生理盐水对照组(NS组),每组20只。MPTP组小鼠皮下注射MPTP(14mg/kg每2h一次,共4次),NS组小鼠皮下注射等量的生理盐水。第7天取鼠脑采用RT-PCR方法观测黑质BDNF mRNA,免疫组织化学方法观察黑质BDNF免疫反应性的表达。结果 EE+NS组与SE+NS组比较,黑质BDNF mRNA表达增加(P<0.01);黑质区BDNF-ir阳性细胞数及矫正光密度(COD)值均增加(P<0.01)。SE+MPTP组与SE+NS组比较,黑质BDNF mRNA表达下降(P<0.001);黑质区BDNF-ir阳性细胞数及COD值均下降(P<0.01)。EE+MPTP组与SE+MPTP组比较,黑质BDNF mRNA表达增加(P<0.01);黑质区BDNF-ir阳性细胞数及COD值均增加(P<0.01)。结论 EE能增加SAMP8小鼠黑质区BDNF mRNA及BDNF-ir的阳性表达,降低MPTP对SAMP8小鼠黑质的损伤,对黑质有保护作用。
Objective To observe the effects of enriched environmen（EE） on brain-derived neurotrophic factor（BDNF） in substantia nigra（SN） of senescence-accelerated prone mice,so as to explore the possible mechanism of EE in alleviating MPTP induced damage and in protecting dopaminergic neurons in the SN.Methods Totally 80 3-month old female SAMP8 mice were averagely assigned to EE and standard environment（SE） groups at random.After three months,the mice in each group were further divided into 2 subgroups at random：MPTP group and NS group（n = 20）.The MPTP groups received subcutaneous injection of MPTP,and the NS mice were treated with an equal volume of NS.At the seventh day,the mice were sacrificed for RT-PCR and immunohistochemistry staining.RT-PCR was used to examine BDNF mRNA expression and immunohistochemistry staining was used to examine BDNF-ir expression.Results Compared with SE ＋ NS mice,EE ＋ NS mice had significantly increased BDNF mRNA expression（P 〈 0.01）,and the number of BDNF-ir cell and COD values in SN were also significantly increased（P 〈 0.01）.Compared with SE ＋ NS mice,SE ＋ MPTP mice showed significantly decreased BDNF mRNA expression（P 〈 0.001）,and the number of BDNF-ir cells and the COD values in SN of mice were significantly decreased（P 〈 0.01）.Compared with SE ＋ MPTP mice,EE ＋ MPTP mice showed increased BDNF mRNA expression（P 〈 0.01）,and the number of BDNF-ir cells and the COD values in SN were significantly increased（P 〈 0.01）.Conclusion EE can increase BDNF mRNA expression and BDNF-ir cell number in SN of SAMP8 mice,alleviating MPTP-induced SN damage.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2010年第11期1179-1183,共5页
Academic Journal of Second Military Medical University
基金
河北省自然科学基金(C2009001242)
河北省卫生厅课题(20090338)~~
