摘要
目的 对家族聚集性高血压心血管疾病标志物功能分类基因表达进行研究,以筛查候选基因.方法 选择3代以上直系亲属均有原发性高血压病史的患者作为实验组,并以健康志愿者作为对照组.采用Oligo GEArray基因芯片技术检测外周血液心血管疾病标志物基因的表达,以阳性标准值/阴性标准值＞2.0,或≤0.5和＞0的基因为差异表达基因.结果 与对照组相比,实验组心血管疾病标志物功能分类基因表达上调的基因共有10条,涉及脂质代谢、免疫应答相关分子、细胞黏附分子、细胞外分子及凝血基因,包括载脂蛋白E（ApoE）、上皮V样抗原-1（EVA-1）、γ-干扰素（IFN-γ）、白细胞介素-1β（IL-1β）、IL-8、整连蛋白-β（ITGB-1）、基质金属蛋白酶-9（MMP-9）、核转录因子-κB（NF-κB）、血小板-内皮细胞黏附分子-1（PECAM-1）、P-选择素（SEL-P）;下调基因3条,包括凝血因子-Ⅲ（F-Ⅲ）、血凝素样氧化型低密度脂蛋白受体-1（LOX-1）、丝氨酸蛋白酶抑制因子-1（SERPINE-1）.结论 家族聚集性高血压涉及多种心血管疾病标志物基因,尤其与凝血相关的F-Ⅲ和与细胞外分子蛋白酶抑制剂、凝血相关的SERPINE-1两个基因有关.
Objective To explore the cardiovascular diseases marker gene expression profile of the familial aggregation hypertension patients,and to screen differentially expressed genes.Methods The patients who had directly related family members for more than three generations suffering from hypertension were selected as experiment group, and healthy individuals as control group.Oligo GEArray gene chip technique was used to detect the expression of cardiovascular diseases marker gene in peripheral blood.The ratio of positive/negative standard value ＞2.0, or ≤0.5 and ＞0 was indentified as differential gene.Results Compared with control group,there were 10 up-regulated differential genes in experiment group, composing genes involved in lipid metabolism, immune response-related molecules, cell adhesion molecules, extracellular molecules and coagulation,including apolipoprotein E （ApoE）,epithelial V-like antigen-1 （EVA-1）,interferon-γ （IFN-γ）, interleukin-1β （IL-1β）, IL-8, integrin-β1 （ITGB-1）, matrix metalloproteinase-9 （MMP-9）, uclear factor-κB （NF-κB）, platelet endothelial cell adhesion molecule-1 （PECAM-1）,selectin-P（SEL-P）.There were 3 down-regulated genes, including coagulation factors- Ⅲ （F- Ⅲ ）, lectin-like oxidized low density lipoprotein receptor-1 （LOX-1）, and serine protease inhibitor-1 （SERPINE-1）.Conclusion This study suggested that familial aggregation hypertension related to a variety of gene markers of cardiovascular disease, especially elements concerning coagulation and extracellular protease inhibitor-related genes.
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2010年第11期684-687,共4页
Chinese Critical Care Medicine
基金
甘肃省科学事业费研究资助项目（QS061-C33-15）
关键词
高血压
家族聚集性
功能分类基因芯片
差异表达基因
Hypertension, familial aggregation
Functional classification gene array
Differentially expressed gene
