摘要
以文心兰试管苗叶片为材料,在(25±1)℃,光照强度24～30μmol/m2.s,光照时间10 h/d下研究类原球茎的诱导和增殖技术。结果表明:①蔗糖适于作为类原球茎诱导的碳源,类原球茎诱导率52%,单位叶片类原球茎2.725个;②附加3～5 mg/L的Ad处理有利于类原球茎诱导(诱导率57%,单位叶片类原球茎数量3.725～4个);③6-BA是影响文心兰类原球茎增殖的主导因子,中等浓度的6-BA(3.0 mg/L)有利于类原球茎的增殖,增殖率(15.29)极显著高于其它水平;④培养基1/2MS+6-BA 3.0 mg/L+2,4-D 1.0 mg/L+NAA 0.4 mg/L+蔗糖20 g/L最有利于文心兰类原球茎的增殖,增殖率最高(15.95),极显著高于其它处理组合。
Using leaves of tissue cultured seedlings as materials,inducing and propagating of protocorm-like bodies（PLBs） were studied under illumination intensity of 24～30 μmol/m2.s and period of 10 h/d.Results indicated that,1） sugar was the carbon sources suitable for protocorm-like bodies inducing,with the inducing rate being 52% and 2.725 PLBs per leaf being induced;2） Addition of 3-5 mg/L AD was helpful to the induction of PLBs,with the inducing rate being 57% and 3.725-4 PLBs per leaf being induced;3） 6-BA was the main affecting factor in the propagation of PLBs,and medium concentration（3.0 mg/L） was suitable for the propagation of PLBs,with an significant higher propagating rate（15.29） than other treatments.4） 1/2 MS＋6-BA 3.0 mg/L ＋2,4-D 1.0 mg/L ＋NAA 0.4 mg/L ＋sugar 20 g/L was the best for propagation of PLBs,with the highest propagation rate（15.95） among all treatments.
出处
《热带作物学报》
CSCD
2010年第9期1464-1468,共5页
Chinese Journal of Tropical Crops
基金
广东省高等学校本科特色专业建设点
广东海洋大学大学生创新实验CXSY200911
关键词
文心兰
类原球茎
诱导
Oncidium
Protocorm-like bodies
Induction
