摘要
目的探讨两种中药方剂对MRL/lpr狼疮小鼠血浆中的B淋巴细胞刺激因子(Blys)、IL-10的影响,为中药及中西医结合治疗红斑狼疮提供实验和理论基础。方法将34只MRL/lpr狼疮小鼠随机分为6组。A组:中药方1组(清热凉血、祛风除湿方);B组:中药方2组(养阴清热活血、滋补肝肾方);C组:泼尼松组;D组:中药方1加泼尼松组;E组:中药方2加泼尼松组;F组:空白对照组。ELISA法测小鼠血浆中Blys、IL-10的含量。结果各药物组治疗后的Blys水平均显著低于F组(P<0.01)。D、E组的Blys水平与A、B、C组、激素组差异有统计学意义(P<0.01)。A、B组与F组比较,IL-10水平差异无统计学意义(P>0.05)。D、E组IL-10水平与F组比较,差异有统计学意义(P<0.01)、与C组相比差异无统计学意义(P>0.05)。结论中药方剂与糖皮质激素均对MRL/lpr狼疮小鼠有治疗作用,中西医综合治疗疗效优于单纯使用中药或糖皮质激素,能显著降低Blys水平。
Objective To investigate the effects and the mechanisms of two lupus recipes including lupus recipe 1（LR1）and lupus recipe 2（LR2）which was traditional Chinese medicine（TCM）to the MRL/lpr mice.to provide the data and basis for the treatment of systemic lupus erythematosus with traditional Chinese medicine and combined Chinese-Western medicine.Methods 34 MRL/lpr mice were randomly divided into 6 different groups：Group A was LR1.Group B was LR2.Group C was prednisone.Group D was LR1＋prednisone.Group E was LR2＋ prednisone.Group F was negative control.Serum Blys and IL-10 were measured by ELISA.Results The change of Blys：The level of Blys in group A,B,C,D and E were all lower than that of group F（P〈0.01）.Compared with group A,B or C,the group D and E were all significant lower（P〈0.01）.The change of IL-10：Compared with the group F,the group A and B were no significant difference（P〈0.05）.Compared with the group D and E,the group F was significant difference（P〈0.01）,and group C was no difference（P〈0.05）.Conclusion LR and prednisone should be effective to the treatment of MRL/lpr mice.Both of them can decrease the level of Blys.The TCM and glucocorticoid used in combination was superior to that used alone.
出处
《重庆医学》
CAS
CSCD
北大核心
2010年第23期3214-3216,共3页
Chongqing medicine
基金
重庆市卫生局课题(2007-1-7)
