摘要
高致病性猪蓝耳病病毒(HP-PRRSV)(JX-EF112445)核苷酸序列与经典猪蓝耳病病毒(LP-PRRSV)(U87392)核苷酸序列,设计两对特异性引物,建立了Hp-PRRSV和LP-PRRSV二重RT-PCR快速检测体系。应用此体系成功对1498份组织/血清进行了准确检测。同时,分别与商品化的HP-PRRSV RT-PCR检测试剂盒和LP-PRRSV检测试剂盒进行比对试验,结果吻合率达100%,该方法特异性好、敏感性高、克服了对两种病原进行分别检测所带来的耗时、耗试剂、增加污染几率等风险,为蓝耳病的快速诊断和流行病学调查奠定了基础。
Based on the mutation characteristic of genome sequence of highly pathogenic porcine reproductive and respiratory syndrome virus （HP- PRRSV） ,and low pathogenic porcine reproductive and respiratory syndrome virus （LP-PRRSV） , two pair primer s were designed , a rapid duplex RT-PCR method for detecting HP-PRRSVand LP-PRRSV were developed. 1498 samples were detected with the eatablished duplex RT-PCR assay.Compared with commercial kits, the results indicated sensitivity high and specificity good. And the methods avoid the risk of time, reagent consuming , the pollution.the assay laid solid foundation for PRRSV detection and epidemiology.
出处
《畜牧市场》
2010年第12期10-12,共3页
stockbreeding market
关键词
高致病性猪蓝耳病
猪蓝耳病
二重RT-PCR
Highly Pathogenic Porcine reproductive and respiratory syndrome virus
low Pathogenic Porcine rep roductive and respiratory syndrome virus
duplex RT-PCR
detection
