摘要
目的 探讨c-Jun氨基末端激酶（JNK）抑制剂减轻应激性溃疡大鼠胃黏膜损伤的可能性.方法 将25只雄性SD大鼠随机等分为对照组、致伤1、6、12 h组、抑制剂组.采用免疫组织化学、Western blot方法检测致伤后胃黏膜磷酸化c-Jun氨基末端激酶（p-JNK）的表达与分布.同时,检测各时间点大鼠胃液pH值、胃黏膜血流量、胃黏膜损伤指数（UI）,并观察胃黏膜大体及光镜下组织病理学变化.结果致伤组p-JNK表达较对照组有明显增加.致伤12 h后,应激性溃疡损伤部位组织上皮细胞p-JNK呈强阳性反应.抑制剂组损伤（15.60±2.70）较致伤12 h组（24.20±2.39）轻（P〈0.01）.致伤12 h组（2.12±0.14,26.90±2.60）、抑制剂组（1.99±0.08,26.66±0.73）胃酸PH值和胃黏膜血流量较对照组（3.44±0.03,35.46±4.48）明显降低（P〈0.01）,但这两组间差异无统计学意义（t=0.082,P〉0.05;t=0.26,P〉0.05）.结论 在液压打击致应激性溃疡大鼠中,JNK信号通路的激活参与了应激性溃疡的发生,其抑制剂能减轻应激性溃疡大鼠胃黏膜的损伤.
Objective To investigate the possibilities of c-Jun N-terminal kinase （JNK） inhibitor to reduce gastric mucosal injury in rats with stress ulcer.Methods Twenty-five male SD rats were randomly divided into five groups：control group,1,6,12 hours injured group,inhibitor of JNK intervention group.Immunohistochemistry and Western blotting were used to detect the expression and distribution of gastric mucosa p-JNK after injuried.Simultaneously,in each group of rats,gastric PH value,gastric mucosal blood flow,gastric mucosal injury index （UI） were measured,and the histology of gastric mucosa was observed.Results Compared with the control group,p-JNK in the injured groups were significantly expressed.Injured after 12 hours,p-JNK of epithelial cells in stress ulcer rats was strongly positive reaction.The injury of inhibitor group （ 15.60 ±2.70） was lighter than the 12 hours injured group （24.20 ±2.39） （ P 〈 0.01 ）.Between the 12 hours injured group （2.12 ± 0.14,26.90 ± 2.60） and inhibitor group （ 1.99 ±0.08,26.66 ±0.73）,gastric PH value and gastric mucosal blood flow were lower than the control group （ 3.44 ± 0.03,35.46 ± 4.48 ） obviously （ P 〈 0.01 ）,but there was no significant difference be tween the two groups （ t = 0.082 ,P 〉 0.05 ; t = 0.26 ,P 〉 0.05 ） .Conclusion In the stress ulcer rats induced by fluid-percussion model,the activation of JNK signaling pathway may initiate and its inhibitors can reduce the gastric mucosal injury.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2010年第12期1895-1897,共3页
Chinese Journal of Experimental Surgery
基金
军队＂十一五＂计划资助项目（06G54）
