摘要
目的:探讨重度子痫前期孕妇外周血胎儿有核红细胞(FNRBC)水平及其红细胞免疫黏附(RCIA)调节因子活性的变化及相关性。方法:采用病例对照研究方法,选择重度子痫前期孕妇34例为研究组,同期18例正常孕妇为对照组,采用单密度梯度离心法,富集计算FNRBC数量;同时采用ELISA法测定孕妇血清RCIA促进因子和抑制因子活性及红细胞受体花环率(RBC-C3bRR)和红细胞免疫复合物花环率(RBC-ICR),分析指标变化及相关性。结果:研究组孕妇外血中FNRBC数量较对照组显著升高(P<0.05);研究组RBC-C3bR花环促进率(RFER)与对照组相比差异无统计学意义(P>0.05),而RBC-C3bR花环抑制率(RFIR)明显高于对照组(P<0.05);研究组RBC-C3bRR明显低于对照组(P<0.05),而RBC-ICR则明显高于对照组(P<0.05)。研究组FNRBC水平与RBC-C3bRR呈负相关(r=-0.568,P<0.05)、而与RFIR、RBC-ICR呈正相关(r=0.673,P<0.05;r=0.712,P<0.05)。结论:重度子痫前期孕妇外周血中FNRBC数量增多能引起患者红细胞免疫黏附抑制因子活性增加,红细胞免疫功能低下,胎儿物质向母血的渗漏增多,机体免疫调控失衡和紊乱,均可能是重度子痫前期免疫学发病机制中的重要环节。
Objective：To investigate the changes and correlation of maternal peripheral fetal nucleated red blood cells（FNRBC） levels and activity of plasma erythrocyte immune adherent regulatory factor in pregnant women with severe pre-eclampsia.Methods：In a case-control study,34 pregnant women with severe preeclampsia as study group and 18 healthy pregnant women as control group were examined. FNRBC were enriched by single density gradient centrifugation and counted. Plasma E rosette enhanced rate of RBC（RFER） and E rosette inhibitive rate of RBC（ RFIR）,rosette rate of C3b receptor of RBC （RBC-C3bRR） and rosette rate of immune compound of RBC （RBC-ICR） were detected by ELISA. The changes of index and correlation were analyzed. Results：The number of FNRBC in peripheral blood was significantly elevated in study group compared with control group（P〈0.05）. RFER was no different in study group compared with control group（P〈0.05）.RFIR was significantly elevated in study group compared with control group（P〈0.05）. RBC-C3bRR was significantly lower in study group compared with control group （P〈0.05）,but RBC-ICR was significantly elevated in study group compared with control group （P〈0.05）. The level of FNRBC in study group was negative correlation with RBC-C3bRR （r=-0.568,P〈0.05）,but positive correlation with RFIR （r=0.673,P〈0.05） and RBC-ICR（r=0.712,P〈0.05）. Conclusions：The increased numbers of peripheral FNRBC in the pregnant women with severe pre-eclampsia may be related to increase of activity of erythrocyte immune adherent inhibit factor,decrease of erythrocyte immune adherent function,increase of fetal-maternal leakage,Organism immunoregulation disbalance and disorder may be one of the major links in the pathogenesis of immunology of severe pre-eclampsia.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2010年第11期826-828,I0001,共4页
Journal of Practical Obstetrics and Gynecology
基金
山东省青岛市科技局基金资助项目(项目编号:Kzd-12)
关键词
子痫前期
妊娠
有核红细胞
胎儿
红细胞免疫
Pre-eclampsia
Pregnancy
Nucleated red blood cells
Fetus
Erythrocyte immunity
