摘要
为研究多种副粘病毒融合蛋白（Ｆ）及其与血凝素－神经氨酸酶（ＨＮ）的相互作用关系，采用Ｔ７ＲＮＡ聚合酶表达系统，将质粒ＤＮＡ及载有各种Ｆ或ＨＮ基因的重组质粒ＤＮＡ于真核细胞内进行表达，然后常规检测表达产物的功能情况。结果显示，每种病毒Ｆ单独表达不足以引起细胞融合，只有与同源性ＨＮ共同表达时，才能引起细胞融合；与异源性ＨＮ共同表达时，则不能引起细胞融合。提示副粘病毒Ｆ的细胞融合作用需要ＨＮ的辅助，并具有极强的特异性。
Two kinds of glycoproteins, hemagglutininneuraminidase(HN) and fusion(F) proteins, are enfolded in the envelopes of paramyxoviruses and the infected cells. In order to know the mechanism of cell fusion mediated by F protein, the expressions and functions of each F protein, coexpression with HN and their interactions with homologous and heterologous HN proteins were studied. Plasmid DNAs containing different F or HN genes were expressed in eukaryocytes under T7 RNA polymerase expression system and then biological functions of expressed products were assayed with common Gimsa staining. The results showed that when each F itself was expressed, there was no fusion when F and its heterologous HN coexpressed. These results proved that the function of cell fusion mediated by F is very specific. The reasons for the specificity were widely discussed.
出处
《山东医科大学学报》
1999年第2期117-119,共3页
Acta Academiae Medicinae Shandong
基金
国家自然科学基金
