摘要
目的:观察沙利度胺对恶性黑色素瘤(恶黑)生长及血管生成模式的影响,为对其治疗提供实验依据。方法:B16黑色素瘤移植瘤模型C57小鼠35只,随机分为实验组20只,对照组15只,分别给予沙利度胺及0.5%羧甲基纤维素钠溶液灌胃。计数肿瘤组织中内皮依赖性血管、马赛克血管和血管生成拟态数目及肿瘤细胞坏死情况,并进行血管内皮细胞生长因子(VEGF)、核因子(NF)-κB、基质金属蛋白酶(MMP)-2、MMP-9和增殖细胞核抗原(PCNA)的免疫组化染色。结果:给药9d后,实验组小鼠的肿瘤体积明显小于对照组(P<0.05)。实验组内皮依赖性血管、马赛克血管和血管生成拟态数量显著低于对照组(P<0.05),且实验组VEGF、NF-κB、PCNA、MMP-2及MMP-9的表达也明显低于对照组(P<0.05)。实验组肿瘤组织中坏死细胞数明显高于对照组(P<0.05)。结论:沙利度胺可以抑制恶黑血管生成,促进肿瘤细胞的坏死,抑制肿瘤的生长。
Objective：To investigate the influence of thalidomide on the tumor growth and angiogenesis of melanoma,and provide experimental evidence for clinical treatment of melanoma.Methods：Thirty-five C57 mice of the melanoma transplanted model were lavaged by thalidomide and 0.5% carboxymethyl cellulose（CMC）.The vasculogenic mimicry,mosaic vessel,endothelial dependent vessel and necrosis ratio of tumor were measured by HE staining.The expression levels of matrix metalloproteinases（MMP）-2,MMP-9,vascular endothelial growth factor（VEGF）,nuclear factor（NF）-κB and proliferating cell nuclear antigen（PCNA） were examined by immunohistochemistry.Results：The tumor volume was significantly decreased in experiment group 9 days after treatment compared with that of control group（P 0.05）.The count values of vasculogenic mimicry,mosaic vessel and endothelial dependent vessel were significantly decreased in experiment group compared with those of control group（P 0.05）.The necrosis ratio of tumor was increased in experiment group compared with that of control group（P 0.05）.The expression levels of MMP-2,MMP-9,VEGF,NF-κB and PCNA of tumor tissues were significantly decreased in experiment group compared with those of control group（P 0.05）.Conclusion：Thalidomide could inhibit angiogenesis,promote tumor cell necrosis and inhibit tumor growth in melanoma.
出处
《天津医药》
CAS
北大核心
2010年第12期1083-1086,共4页
Tianjin Medical Journal
