摘要
目的:研究恰玛古蜜膏和恰玛古粉对小鼠免疫功能的影响。方法:测定小鼠耳廓肿胀度,观察对二硝基氯苯诱导小鼠迟发型超敏反应的影响;通过紫外分光光度法测定观察小鼠单核巨噬细胞对血流中碳粒的清除能力;紫外分光光度法测定小鼠血清中IgM抗体水平;镜检计数观察小鼠腹腔巨噬细胞吞噬鸡红细胞的能力。结果:恰玛古蜜膏14g/kg剂量与恰玛古粉12g/kg剂量能明显抑制二硝基氯苯诱导的小鼠迟发型超敏反应、促进小鼠血清中IgM抗体的生成,恰玛古粉6g/kg和12g/kg剂量能明显提高小鼠单核巨噬细胞对血流中碳粒的清除能力、提高小鼠腹腔巨噬细胞吞噬鸡红细胞的能力。结论:恰玛古蜜膏和恰玛古粉均可调节机体的免疫功能,恰玛古粉的作用略优于恰玛古蜜膏。
Objective： To study the role of Brassica campestris L.ointment and B.campestris L.powder on immune function in mice.Method： Through the delayed type hypersensitivity（DTH） induced by DNCB determine umefaction of ear;The influence on carbongranular clearance ability of mononuclear macrophage in mice was determined by ultraviolet spectrophotometry;the IgM level was determined by ultraviolet spectrophotometry;the ability of englobing red blood cell of chicken of peritoneal macrophage in mice abserve by microscopic examination.Results： DTH induced by DNCB,the level of IgM were restrained by the 14g/kg dosage group of B.campestris L.ointment and the 12g/kg and 6g/kg dosage group of B.campestris L.powder;the carbongranular clearance ability of mononuclear macrophage,the ability of englobing red blood cell of chicken of peritoneal macrophage in mice were enhanced significantly by the 12g/kg and 6 g/kg dosage group of B.campestris L.powder.Conclusion： B.campestris L.ointment and B.campestris L.powder have effect on improving the immunological function of miceand,and the effect of superior on B.campestris L.powder.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第1期141-143,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
乌鲁木齐市人才工程计划项目(No.P101310002)~~
关键词
恰玛古
小鼠
免疫调节
Brassica campestris L.
Mice
Immune function
