摘要
根据棉花黄萎病菌（Ｖｅｒｔｉｃｉｌｌｉｕｍ ｄａｈｌｉａｅ）核糖体基因ＩＴＳ区段的碱基编码序列，设计合成了１对为２６ ｂｐ 的 ＰＣＲ 特异扩增引物（引物１： ５′ＣＡＴＣＡＧＴＣＴＣＴＣＴＧＴＴＴＡＴＡＣＣＡＡＣＧ， 和引物２：３′ＣＧＡＴＧＣＧＡＧＣＴＧＴＡＡＣＴＡＣＴＡＣＧＣＡＡ），进行了大丽轮枝病菌 ＰＣＲ 特异扩增试验。试验结果表明：本试验设计合成的这对引物，能对大丽轮枝菌全基因组 ＤＮＡ 和人工接种棉花黄萎病病株组织特异地扩增到大丽轮枝菌核糖体基因ＩＴＳ区段的３２４ ｂｐ 分子片段，该对引物可用于棉花黄萎病的分子鉴定和分子监测。本试验结果对棉花黄萎病的早期诊断和病原菌的检测和监测有潜在的应用价值。
A pair of PCR primers(26 bp) were designed according to the ITS sequence of rRNA gene of Verticillium dahliae. (P1: 5′CATCAGTCTCTCTGTTTATACCAACG, P2: 3′CGATGCGAGCTGTAACTACTACGCAA). Results from assays done with these two primers showed that a 324 bp fragment of rRNA gene was amplified from genomic DNA of V. dahliae and diseased plant tissue inoculated with V. dahliae. These two primers will be useful for molecular identification and detection of cotton wilt. Results from this study are very important to the diagnose of cotton wilt by V.dahliae in the early stage,and study of pathogenicity and disease forecast.
出处
《植物病理学报》
CAS
CSCD
北大核心
1999年第3期250-255,共6页
Acta Phytopathologica Sinica
