摘要
本试验比较研究了富硒益生菌(Selenium-enriched probiotics,SP)和亚硒酸钠(Sodium selenite,SS)对蛋鸡肝脏、肾脏和心肌硒含量及其抗氧化能力的影响。选取800羽健康罗曼蛋鸡,随机均分为8组,将2硒源分别以3个硒水平(0.2、0.5和1.0mg·kg-1)添加到基础日粮中组成6种试验日粮,同时将益生菌添加到基础日粮中作为益生菌对照,基础日粮作为空白对照,进行为期28d的饲养试验。在试验结束时,杀鸡取肝脏、肾脏和心肌分别进行硒含量及其谷胱甘肽过氧化物酶(GSH-Px)活性、过氧化氢酶(CAT)活性、超氧化物歧化酶(SOD)活性和总抗氧化能力(T-AOC)的测定。结果表明,补硒能提高蛋鸡肝脏、肾脏、心肌的硒含量以及GSH-Px、CAT、SOD的活性和T-AOC(P<0.05);且随着硒添加水平的升高,肝脏、肾脏和心肌的硒含量、GSH-Px活性均有显著升高(P<0.05);添加SP在提高肝脏和心肌硒含量的能力上和在提高肝脏、肾脏和心肌的GSH-Px活性、CAT活性以及肾脏的SOD活性和T-AOC的能力上要优于或显著优于SS。结果提示,补充硒能提高蛋鸡组织硒含量及其抗氧化能力,特别提倡添加低中水平(0.2和0.5mg·kg-1)SP源硒。
This experiment was conducted to compare the effects of Selenium-enriched probiotics（SP） and sodium selenite（SS） on selenium contents and the antioxidant capacity of hen tissues.Both Se sources were added into basal diet at 0.2,0.5 and 1.0 mg·kg-1 of Se to make six types of experiment diets.At the same time,probiotics was added into basal diet as the probiotics control,and basal diet served as the blank control.Eight hundred healthy Rohman laying hens were randomly and equably allocated into eight groups and were investigated for 28 days.At the end of the experiment,hens were slaughtered,and liver,kidney and cardiac muscle were sampled for Se content and GSH-Px,CAT,and SOD activity and T-AOC value assay.The results showed that supplemental Se significantly increased Se contents and the activity of GSH-Px,CAT and SOD and T-AOC value in liver,kidney and cardiac muscle of hens.With supplemental Se level increase,Se contents and the activity of GSH-Px in liver,kidney and cardiac muscle all were significantly increased.Supplemental SP had more ability than SS to increase Se content in liver and cardiac muscle,and to increase the activity of GSH-Px and CAT in liver,kidney and cardiac muscle,and the activity of SOD and T-AOC value in kidney.It was concluded that supplementation of Se can increase Se contents and the antioxidant capacity of hen tissues.Especially,advocating to supplement SP at a lower or medium Se level,0.2 or 0.5 mg·kg-1.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第12期1605-1613,共9页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金项目(30671547
30871892)
博士点科学基金项目(20070307005)资助
关键词
富硒益生菌
亚硒酸钠
蛋鸡
硒含量
抗氧化能力
selenium-enriched probiotics
sodium selenite
hen
selenium contents
the antioxidant capacity
