摘要
目的:探讨辛伐他汀对体外培养的成骨细胞系MG63细胞功能的影响,为辛伐他汀用于牙槽骨缺损再生的研究提供理论依据。方法:MG63成骨细胞经辛伐他汀处理后,分别用MTT法、荧光实时定量PCR与细胞微趋化试验检测其功能。采用SPSS14.0软件包进行单因素方差分析。结果:10-9mol/L与10-8mol/L浓度辛伐他汀可轻微促进MG63细胞的增殖;而10-6mol/L与10-7mol/L浓度的辛伐他汀可显著提高骨保护素与骨钙素基因的表达,差别具有统计学意义;所有浓度的辛伐他汀均对MG63细胞的迁移能力具有明显抑制作用。结论:辛伐他汀可促进骨质缺损的再生,其促进作用主要是通过成骨相关基因的表达增加来实现的。
PURPOSE：To investigate the effect of simvastatin on the function of MG63 cell line.METHODS：The function of proliferation,osteoprotegerin（OPG） and osteocalcin（OC） gene expression,and migration of MG63 cells were detected with MTT,fluorescent real-time PCR and micro chemotaxis,respectively.The data was analyzed using ANOVA followed by Tukey test with SPSS14.0 software package.RESULTS：10-9mol/L and 10-8mol/L concentrations of simvastatin slightly promoted MG63 cell proliferation;10-7mol/L and 10-6mol/L concentrations of simvastatin greatly enhanced the OPG and OC mRNA expression;All concentrations of simvastatin had an inhibitory effect on MG63 cell migration.CONCLUSION：Simvastatin could promote bone defect regeneration by enhancing the bone-related genes expression.
出处
《上海口腔医学》
CAS
CSCD
2010年第6期658-662,共5页
Shanghai Journal of Stomatology
