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水产致病性嗜水气单胞菌耐药性比较与分析 被引量:42

Comparison and Analysis of Drug Resistance to Morbific Bacterium Aeromonas hydrophila Isolated from Aquatic Animals
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摘要 对15株分离自甲鱼、草鱼、鲫鱼、石蛙、团头鲂和三角鲂的嗜水气单胞菌,用K-B纸片法对18种抗生素进行药敏试验后,用NCCLS标准进行判断,结果输入WHONET5.4耐药监测软件,比较15株菌的耐药水平及18种抗生素的耐药率,并对四环素和强力霉素表现出来的交叉耐药性进行分析,结果表明,15株嗜水气单胞菌耐药现象较为严重,恩诺沙星、四环素、强力霉素和利福平耐药率均超过40%,WHONET5.4散点图表明四环素和强力霉素有较为密切的交叉耐药现象。 Antimicrobial susceptibility tests of 15 Aeromonas hydrophila strains isolated from different aquatic animals including Chinese soft shelled turtle(Trionyx sinensis),grass carp(Ctenophyaryngodon idella),crucian carp(Carassius auratus),frog,Megalobrama amblyaphala and M.terminaiis were carried out on Mueller Hinton agar by the Kirby Bauer disc diffusion method according to NCCLS guidelines.The results were analysed by WHONET5.4.Resistance of Aeromonas hydrophila to ENR,DOX,LET,and RIF was found to be higher than 40 percents.LET and DOX scatter plot indicated that they had relatively Cross Resistance.
出处 《水产科学》 CAS 北大核心 2011年第1期42-45,共4页 Fisheries Science
基金 杭州市科技发展计划项目(20091832B04)
关键词 嗜水气单胞菌 耐药性 WHONET5.4 Aeromonas hydrophila drug resistance WHONET5.4
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参考文献4

  • 1李景云,许明哲,马越,胡昌勤,金少鸿.细菌耐药性监测数据处理软件WHONET 5.3简介[J].中华检验医学杂志,2004,27(11):805-806. 被引量:9
  • 2NCCLS. National committee for clinical laboratory standards. Performance standards for antimicrobial disk susceptibility test-tenth edition: approved stand- ard M02-A10[S]. Wayne, Pennsylvania, USA: NC- CLS, 2009.
  • 3Clinical and Laboratory Standards Institute. Methods for Antimierobial Dilution and Disk Susceptibility Tes- ting of Infrequently Isolated or Fastidious Bacteria: Approved Guideline. CLSI Document M45-P FS3. Wayne, PA, USA : CLSI, 2005.
  • 4周贵民,张有江,张淑兰,许淑珍,张秀珍,宣天芝,陈民钧,徐英春.北京4家医院对常用较新的16种抗生素的敏感性监测[J].中华微生物学和免疫学杂志,1995,15(4):219-224. 被引量:76

二级参考文献4

  • 1Stelling JM, O′Brien TF. Surveillance of antimicrobial resistance: the WHONET program. Clin Infect Dis. 1997,24 Suppl 1:S157-68.
  • 2NCCLS. Analysis and presentation of cumulative antimicrobial susceptibility test data;approved guideline. NCCLS document M39-A[ISBN 1-56238-463-5]. NCCLS,940 West valley road, suite 1400, Wayne, pennsylvania19087-1898 USA, 2002.
  • 3NCCLS. Perfomance standards for antimicrobial disk susceptibility tests;approved standard-eighth edition. NCCLS document M2-8[ISBN 1-56238-485-6]. NCCLS,940 West valley road, suite 1400, Wayne, pennsylvania19087-1898 USA, 2003.
  • 4NCCLS. Perfomance standards for antimicrobial susceptibility testing; fourteeth informational supplement.NCCLS document M100-S14[ISBN 1-56238-516-X]. NCCLS,940 West valley road, suite 1400, Wayne, pennsylvania19087-1898 USA, 2004.

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