摘要
采用高效液相色谱法测定南海海域多种水产品中软骨藻酸(DA)的含量。样品粗提液通过LC-SAX强阴离子柱固相萃取净化,在乙腈-0.1%三氟乙酸水溶液(体积比为19:81)流动体系下进行定性分析,以外标法进行定量。结果表明,DA在1 mg/L～25.0 mg/L范围内有良好的线性关系,R=1.0,平均回收率为95.3%;11批次样品中有9批次被检出有DA累积,扇贝和钝齿短桨蟹中的DA累积较高,分别为10.113μg/g和18.202μg/g。
The contents of domoic acid（DA） poisoning in various aquatic products from South China Sea were detected by HPLC.The samples were firstly purified with LC-SAX（strong anion exchange）-SPE（solid phase extraction） and eluted with the mobile phase of acetonitrile-0.1% trifluoroacetate acid（19：81） using external standard method.A good linear range was found within 1-25.0 mg/L（r=1.0）.The average recovery was 95.3%.And DA accumulation was detected in nine of the eleven samples.Higher DA accumulation was found in Chlamys farreri and Thalamita crenata,being of 10.113 μg/g and 18.202 μg/g respectively.
出处
《现代食品科技》
EI
CAS
2011年第1期120-122,116,共4页
Modern Food Science and Technology
