摘要
硫甙及其降解产物异硫氰酸酯、噁唑烷硫酮等是菜籽饼粕中主要的有毒有害物质,是菜籽饼粕饲用的第一限制性因素。利用筛选出的能高效降解菜籽粕硫甙的菌株,研究了混合发酵的不同接种量、不同含水量、不同发酵时间及不同预处理方式对菜籽粕硫甙降解率的影响。结果表明:纳豆芽孢杆菌、枯草芽孢杆菌B3和酿酒酵母以15%的总接种量,等比例接种到经膨化预处理的菜籽粕(含水量为40%)中,发酵72 h,菜籽粕中的噁唑烷硫酮和异硫氰酸酯降解率可达到71.16%、80.25%。
The glucosinolate and its degradation products such as isothiocyanate, oxazolidine and so on are the main poisonous and harmful substances and the first restrictive factor of the rapeseed meal forageuse. The strains with high efficient degradation of glucosinolate were screened, and then the mixed fermentation was carried on. The influence of inoculation quantity ,moisture ,fermentation time and pretreatment ways on the glucosinolate degradation rate was studied. The results indicated that the best conditions of fermentation were as follows： the mixed strains of Bacillus natto, Bacillus subtilis B3 and Saccharomyces cerevisiae inoculated proportionally to the rapeseed meal culture; the total inoculation amount 15%, water content in culture 40%, fermentation time 72 h and the rapeseed meal expanded before fermentation. Under the best conditions, the degradation rates of oxazolidine and isothiocyanate were 71.16% and 80. 25% ,respectively.
出处
《中国油脂》
CAS
CSCD
北大核心
2011年第1期34-37,共4页
China Oils and Fats
基金
科技部科研院所技术开发研究专项资金项目(2008EG234281)
2010年湖北省自然科学基金项目(ZRZ0073)
关键词
菜籽粕
硫甙
固态发酵
混合发酵
rapeseed meal
glucosinolates
solid fermentation
mixed - fermentation
