摘要
目的:建立测定栀子金花丸中盐酸小檗碱和绿原酸含量的毛细管电泳法。方法:采用未涂层弹性融硅石英毛细管柱(60 cm×75μm ID,有效长度52 cm);分别以0.2 mol.L-1磷酸二氢钠溶液+无水乙醇(50∶50)(pH 5.50)、50 mmol.L-1硼砂溶液为运行缓冲液;分离电压依次为21 kV,12 kV;重力进样10 s(高度15 cm);检测波长依次为265 nm,327 nm。结果:盐酸小檗碱和绿原酸浓度分别在11.0～44.0 mg.L-1,3.6～14.4 mg.L-1具有良好的线性关系(r均为0.999 3),平均加样回收率为100.0%,98.1%,方法精密度RSD 1.72%,0.96%(n=6)。栀子金花丸中盐酸小檗碱的平均含量3.39 mg.g-1;绿原酸的平均含量为0.88 mg.g-1。结论:该方法准确、灵敏、快速,可用于栀子金花丸中盐酸小檗碱和绿原酸的质量控制研究。
Objective：To establish the method for determination of berberine hydrochloride and chlorogenic acid in Zhizi jinhuawan by capillary electrophoresis. Method： The separation was performed on a fused silica capil- lary of 60 cm ~ 75 μm ID （52 cm of effective length）. 0.2 moL· L ^- 1 Nail2 PO4 ＋ CH3 CH2 OH （50： 50 ） （ pH 5.50） and 50 mmoL· L ^- 1Na2B4OTwere selected as the running buffers. The separation voltages were 21 kV and 12 kV, gravity injection （10 s, 15 cm）. The detection wavelengths were 265 nm and 327 nm. Result： The concentration ranges of determination for berberine hydrochloride and chlorogenic acid were 11.0-44. 0 mg· L ^- 1 and 3.6-14. 4 mg ~ L-i with linear ralationship （r = 0. 999 3 ）. The average recoveries were 100. 0% and 98. 1% , precisions of the method were 1.72% and 0. 96% （RSD, n = 6）. The average content of berberine hydrochloride was 3.39 mg· g ^- 1 , chlorogenic acid was 0. 88 mg.g ^-1. Conclusion：The method was accurate, sensitive and rapid for the quality control of Zhizi Jinhuawan.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第3期86-89,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省自然科学基金资助项目(5002841)
关键词
毛细管电泳法
栀子金花丸
盐酸小檗碱
绿原酸
capillary electrophoresis
Zhizi Jinhuawan
berberine hydrochloride
ehlorogenic acid
