摘要
利用EST-SSR分子标记技术,选用自主开发的3对EST-SSR引物,对13个大白菜品种的总DNA进行了PCR扩增。结果表明:EST-SSR标记可以体现品种内的一致性、亲本间以及品种间的多态性,具有共显性、稳定性和可重复性的特点;EST-SSR标记可以快速、准确地鉴别大白菜品种的真实性和杂交种纯度,为大白菜种子质量的高效检测提供科学依据。
The seed purity of 13 Chinese cabbage cuhivars by the PCR-base assays with the templates of total DNAs and three EST-SSR primers developed from GenBank Database were identified. The results showed that there were significant polymorphisms among different cultivars or betwwen parents. The co-dominant bands were also found among the hybrids and their parents in our experi- ments. Concludely, the EST-SSR markers could be used to assess genetic purity of Chinese cabbage hybrid seeds and identify different cultivars.
出处
《天津农业科学》
CAS
2010年第6期1-4,共4页
Tianjin Agricultural Sciences
基金
国家973前期研究专项(2009CB126002)
山东省农业科学院创新基金(2007YCX003)
国家科技基本条件平台建设项目(2005DKA21001-38)
关键词
大白菜
种子纯度
鉴定
EST—SSR标记
Chinese cabbage （Brassica rapa L .ssp. pekinensis）
seed purity
identification
EST-SSR markers
