摘要
目的:建立同时测定栀子中栀子苷、西红花苷-Ⅰ和西红花苷-Ⅱ含量的方法,并对14批不同产地栀子药材中3种成分的含量进行测定。方法:采用反相高效液相色谱法。色谱柱为Thermo ODSC1(8250mm×4.6mm,5μm),流动相为甲醇-0.1%磷酸水溶液(梯度洗脱),流速为1.0mL·min-1,检测波长分别为栀子苷238nm、西红花苷-Ⅰ和西红花苷-Ⅱ440nm。结果:栀子苷、西红花苷-Ⅰ、西红花苷-Ⅱ的进样量分别在0.448～5.600、0.142～1.775、0.026～0.325μg范围内与各自峰面积积分值呈良好线性关系,r分别为0.9992、0.9998、0.9999;三者平均回收率分别为99.69%(RSD=2.44%,n=6)、98.26%(RSD=2.62%,n=6)、102.94%(RSD=3.13%,n=6)。结论:本方法简便、准确、重复性好,可同时测定栀子中栀子苷、西红花苷-Ⅰ和西红花苷-Ⅱ的含量。
OBJECTIVE ： To establish a method for the content determination of gardenoside, crocin-Ⅰ and crocin-Ⅱ in Gardenia jasminoides, and to determine the content of 3 components in 14 batches of samples from different producing areas. METHODS： RP-HPLC method was used. A Thermo ODS C18（250 mm×4.6 mm, 5μm） column was used with the mobile phase consisted of acetonitrile-0.1% phosphoric acid （gradient elution） at the flow rate of 1.0 mL·min^-1. The UV detection wavelength was set at 238 nm for gardenoside and 440 nm for crocin-Ⅰ and crocin-Ⅱ , respectively. RESULTS： The linear ranges of gardenoside, crocin-Ⅰ and crocin-Ⅱ were 0.448-5.600 μg（r=0.999 2）, 0.14241.775μg（r=0.999 8） and 0.026-0.325μg（r=0.999 9）, respectively. The average recovery rates of gardenoside, crocin-Ⅰ and crocin-Ⅱ were 99.69% （RSD=2.44%, n=6）, 98.26% （RSD= 2.62 %, n = 6） and 102.94 % （RSD = 3.13 %, n = 6）, respectively. CONCLUSION： The method is sample, accurate and reproducible for the content determination of gardenoside, crocin-Ⅰ and crocin-Ⅱ simultaneously in G. jasminoides.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第7期630-632,共3页
China Pharmacy
