摘要
为了探讨碱性磷酸酶(ALP)基因在大菱鲆(Scophthatmus maximus)体内的表达情况及在细胞水平上三碘甲状腺原氨酸(T3)对碱性磷酸酶(ALP)基因表达量的影响,利用荧光定量PCR法检测了大菱鲆肠、肌肉、脾脏、心脏、鳃、肝脏、肾脏及脑8个不同组织中ALP mRNA的表达情况,并且用0 nmol/L、50 nmol/L、75 nmol/L、100 nmol/L、200 nmol/L 5个不同浓度的T3处理大菱鲆肾细胞(SMKC),采用实时荧光定量PCR法测定T3处理后细胞中ALP mRNA的表达情况。结果表明,ALP mRNA在大菱鲆不同组织中的表达量各不相同,具有组织特异性。心脏组织中ALP mRNA的表达量最高,其次是肝脏组织中和鳃组织中,肌肉组织中ALP mRNA的表达量最少。不同浓度T3处理后大菱鲆肾细胞后,细胞中碱性磷酸酶基因的表达量存在明显的差异,ALP mRNA的表达量随着T3浓度的增加有递增趋势。结论认为,ALP基因在大菱鲆成鱼8个不同组织中均有表达,但其表达量却有明显的差异,具有组织特异性。ALP基因的表达量受甲状腺激素T3正向调控,并有浓度依赖性。
It had been proved that the change of ALP activity had obvious effect on the morphology of osteocyte and digestive function in the metamorphism stage of Japanese flounder（Scophthatmus maximus） larvae.In order to study the expression of alkaline phosphatase（ALP） gene and the effects of triiodothyronine（T3） on the expression of ALP gene in S.maximus,real-time fluorescent quantitative PCR was applied to detect the expression of ALP mRNA in eight different tissues which were intestine,muscle,spleen,heart,gill,liver,kidney,brain and the S maximus＇ kidney cell（SMKC） treated by T3 in five different concentrations（0 nmol/L,50 nmol/L,75 nmol/L,100 nmol/L,200 nmol/L）.The results showed that the expression of ALP mRNA were various in different tissues,and was tissue-specific.The expression of ALP mRNA was the highest in heart,followed by liver and gill,and the lowest was in muscle.There was significant difference on expression of alkaline phosphatase（ALP）gene in SMKC after treated by different concentrations of T3.From the results,it can be concluded that ALP gene was expressed in eight different tissues,but there was significant difference in expression level,with tissue-specific characters.The expression of ALP mRNA had an incremental trend with the increase of T3.It was indicated that expression of ALP gene was positively regulated by T3,and was concentration-dependent.
出处
《中国水产科学》
CAS
CSCD
北大核心
2011年第1期208-213,共6页
Journal of Fishery Sciences of China
基金
国家自然科学基金项目(30271017)
国家教育部博士项目基金(20040264001)
