摘要
目的：探讨抑癌基因Ｐ１６的表达水平与妊娠滋养细胞肿瘤的形成及转移倾向的相关性。方法：采用免疫组织化学方法，检测葡萄胎３０例、侵蚀性葡萄胎２５例、绒毛膜癌２６例及正常绒毛２５例的滋养细胞中Ｐ１６的表达。结果：（１）Ｐ１６在妊娠滋养细胞肿瘤中有较高的表达，阳性率为７４．５％（其中侵蚀性葡萄胎７２．０％，绒毛膜癌７６．９％），明显高于葡萄胎组（２０．０％）和正常绒毛组（１６．０％），差异有高度显著性（Ｐ＜０．０１）；（２）伴有转移的１４例妊娠滋养细胞肿瘤中的Ｐ１６阳性表达率（３５．７％）明显低于３７例不伴转移者（８９．２％），差异有高度显著性（Ｐ＜０．０１）；（３）在复发及癌死亡的病例中Ｐ１６为阴性表达。结论：Ｐ１６基因可能在妊娠滋养细胞肿瘤的形成及转移过程中起调节作用，其表达水平与妊娠滋养细胞肿瘤的转移、恶性程度及预后呈负相关。
Objective: ToinvestigatetherelationshipbetweenP16geneexpressionandoccurrence,metastasisofgestationaltrophoblastictumor. Methods: TheexpressionofP16proteinwasdeterminedinformalinfixed,paraffinembeddedtissuesfrom25normalchoriontissue,30hydatidiformmole,51gestationaltrophoblastictumor(including25invasivehydatidiformmoleand26choriocarcinoma),withimmunohistochemicaltechnique. Results: (1)4outof25normalchoriontissueshowedP16expression(16.0%),while6outof30(20.0%)hydatidiformmolecaseswerepositive,and38outof51(74.5%)casesofgestationaltrophoblastictumorshowedthesamephenomenon,thislastgroupincluding18of25invasivehydatidiformmole(77.0%)and20of26ofchoriocarcinoma(76.9%).TherewassignificantdifferenceinP16expressionbetweengestationaltrophoblastictumorandhydatidiformmoleornormalchoriongroup(P<0.01);(2)Thepositiverate(35.7%)ofP16expressionwassignificantlylowerin14trophoblastictumorcaseswithmetastasisthanin37trophoblastictumorwithoutmetastasis(P<0.01);and(3)Therewasnoexpressionintissuesofreccurrenceanddeathcase
出处
《广东医学院学报》
1999年第2期96-98,共3页
Journal of Guangdong Medical College
关键词
滋养细胞肿瘤
P16基因
基因表达
trophoblastictumor
genes,suppressortumor
geneexpression
immunohistochemistrys. Conclusions: P16genemightparticipateinregulationofoccurrenceandmetastasisprocessingestationaltrophoblastictumor.ThelevelofexpressionofP16isnegativecorrelationwithmetastasisandp
