摘要
采用环状等温扩增技术(Loop-Mediated Isothermal Amplification,LAMP),针对转基因玉米品系MON863中玉米基因组与外源基因结合处设计4条特异性引物,建立MON863的快速检测技术体系,并对时间、温度等反应条件及体系灵敏度、特异性等进行探索。结果表明:该检测体系最适反应温度为63℃,反应时间60 min,灵敏度是常规定性PCR的10倍。该检测方法具有高度的特异性和稳定性,操作方便、简单、省时。
Based on the loop-mediated isothermal amplification （LAMP） reaction, four specific primers were designed according to the six regions of the unique and specific integration junction sequences between the host plant genome DNA and the integrated gene of MON863. The LAMP system and program were optimized. The results showed that the optimum temperature and time was 63 ℃ and 60rain, respectively. The sensitivity of LAMP detection was 0.01%, which was 10 times more sensitive than the conventional PCR. Results indicated that LAMP could provide a sensitive, specific, stable and reliable method for the detection of MON863.
出处
《玉米科学》
CAS
CSCD
北大核心
2011年第1期31-34,共4页
Journal of Maize Sciences
基金
国家科技部国际合作项目(2006DFA32380)
