摘要
目的：对京大戟中大戟二烯醇进行含量测定。方法：采用反相高效液相色谱法,使用Agilent ZORBAX Eclipse XDB-C8（150mm×4.6mm,5μm）色谱柱,流动相为乙腈-水（92∶8）,流速1.0mL·min-1,检测波长210nm,柱温25℃。结果：大戟二烯醇进样量在1.388~10.41μg范围内有良好的线性关系（r=0.9999）,平均回收率（n=6）为97.1%（RSD=1.7%）。结论：本方法能够准确测定京大戟中大戟二烯醇的含量。
Objective：To develop an HPLC method for the determination of euphol content in Radix Euphorbiae Pekinensis.Methods：The chromatographic procedure was carried out on a ZORBAX Eclipse XDB-C8（150 mm×4.6 mm,5 μm）column.The mobile phase was acetonitrile-water（92∶8）at a flow of 1.0 mL·min-1 and the detection wavelength was 210 nm.The temperature of column was 25 ℃.Results：The linear range of euphol was 1.388-10.41 μg（r=0.9999）,and the average recovery（n=6）was 97.1% with RSD of 1.7%.Conclusion：This method is accurate,reliable and sensitive for determination of euphol in Radix Euphorbiae Pekinensis.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第12期2290-2292,共3页
Chinese Journal of Pharmaceutical Analysis
基金
"十一.五"国家科技支撑计划重点项目(No.09XLB3005)
