摘要
目的：对中国药典新增药材辣椒质量标准进行研究。方法：建立药材的来源、性状、显微特征和辣椒素TLC鉴别;采用HPLC方法,色谱柱为DiamonsilTMC18（4.6mm×250mm,5μm）柱,流动相为甲醇-水（50∶50）,流速1.0mL·min-1,检测波长280nm,柱温40℃,测定辣椒中辣椒素和二氢辣椒素的含量并制定含量限度。结果：辣椒的显微鉴别特征性强;辣椒素TLC色谱斑点清晰;辣椒素、二氢辣椒素进样量分别在0.0542~1.3552μg（r=0.9999）和0.0198~0.4952μg（r=0.9999）范围内呈良好的线性关系,平均回收率（n=9）分别为102.0%和98.38%,二者的总平均回收率（n=9）为100.8%。结论：本标准方法简便、可靠、准确,可用于辣椒药材的质量控制。
Objective：To study on the quality standard of new medicine Fructus Capsici Annui.Methods：To establish the origins,characters and microscopic characteristics and capsaicin TLC identification.Using HPLC method,chromatographic column for DiamonsilTMC18（4.6 mm×250 mm,5 μm）column,mobile phase for methanol-water（50∶50）,flow rate 1.0 mL·min-1,detected UV wavelength 280 nm,column temperature 40 ℃,determination capsaicin and dihydrdcapsaicin content and formulate content limit.Results：The strong characteristic microscopic of Fructus Capsici Annui,Capsaicin TLC spots were clear.Capsaicin at 0.0542-1.3552 μg（r=0.9999）and dihydrocapsaicin at 0.0198-0.4952 μg（r=0.9999）was good linear range,and the average recovery（n=9）respectively,both 102.0%,98.38% and the total average recovery（n=9）was 100.8%.Conclusion：This method is simple,reliable and accurate,and can be used for the quality control of Fructus Capsici Annui.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第12期2293-2298,共6页
Chinese Journal of Pharmaceutical Analysis
基金
中国药典2010年版一部标准研究课题(课题编号YD-079)
