摘要
根据基因库中禽巴氏杆菌病原的基因序列（U51470），设计了一对跨幅为488bp的引物，并用这对引物对11个不同血清型的禽巴氏杆菌标准株、9株地方分离株和5株哺孔动物巴氏杆菌菌株及其它6种禽病病原进行了PCR扩增，结果11个血清型的禽源巴氏杆菌和9株禽巴氏杆菌地方分离株均得到了片段大小与实验设计相一致的PCR扩增产物，而对5个血清型的哺乳动物巴氏杆菌和其它6种禽病病原的扩增结果为阴性。该PCR能检出10pg的禽巴氏杆菌DNA模板。
A set of specific primers, XZ21, XZ22 were designed according to the sequences of Genebank No. U51470 of avian Pasteurella Mulutocida. Thirteen reference strains and nine isolates of Pasteurella Multocida and 5 Mammalian pasteurella Multocida were detected by PCR using this set of primers. A avian Pasteurella Multocida-specific 488 base pair DNA product was amplified by these primers from the 13 reference strains and 9 isolates, but not from 5 Mammalian Pasteurella Multocida and 6 other avian pathogenic viruses and bacteria.As little as 10pg of Avian Pasteurella Multocida DNA was detected Using gel electrophoresis.
出处
《中国预防兽医学报》
CAS
CSCD
1999年第6期446-449,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
广西科学基金
