摘要
目的:探讨提取蒲公英多糖的提取最佳工艺和蒲公英多糖对四氧嘧啶(Alloxan)糖尿病小鼠的降糖作用。方法:采用酶提取法、超声波提取法和超声波协同酶提取法,利用正交试验,优化各种提取方法的试验条件;用四氧嘧啶小鼠进行糖尿病模型的建立,用降糖仪测定小鼠的血糖,考察其降糖效果。结果:酶提取法的提取率为:9.203%;超声提取的提取率为:13.98%;超声协同酶法的提取率为:15.83%。结论:提取的蒲公英多糖均有降低血糖的作用(P<0.01)。
Objective： To investigate the optimum technological conditions of Dandelion Polysaccharide and the hypoglycemic effect of its extract in diabetic mice.Methods：Dandelion Polysaccharide was extracted by the methods of enzyme extraction,ultrasonic extraction and ultrasonic-enzyme synergistic extraction.The orthogonal test was used,and the experimental conditions were optimized.The hypoglycemic effect of Dandelion Polysaccharide was investigated by detecting the blood glucose.Results：The extraction rate of enzymes extraction was 9.203%.The extraction rate of ultrasonic extraction was 13.98%.The extraction rate of ultrasonic extraction with enzyme was 15.83%.Conclusion：Dandelion Polysaccharide extracted by all the three methods has hypoglycemic action and Dandelion Polysaccharide extracted by ultrasonic extraction shows the most remarkable hypoglycemic effect.
出处
《黑龙江医药科学》
2010年第6期36-37,共2页
Heilongjiang Medicine and Pharmacy
基金
黑龙江省卫生厅(项目编号:2007-510)
关键词
蒲公英多糖
提取
降糖作用
Dandelion polysaccharide
extraction
hypoglycemic function
